Human Stathmin 2 (STMN2) ELISA Kit | STMN2 elisa kit

Human Stathmin 2 (STMN2) ELISA Kit

Cat. Num. AAA2707690

• Reactivity: Human
• Synonyms: Stathmin 2 (STMN2); Human Stathmin 2 (STMN2) ELISA Kit; SCG10; SCGN10; SGC10; Superior Cervical Ganglion-10; STMN2 elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Stathmin 2 (STMN2).; No significant cross-reactivity or interference between Stathmin 2 (STMN2) and analogues was observed.
• Sequence Length: 179

• Assay Type: Double-antibody Sandwich
• Samples: Tissue Homogenates, Cell Lysates and Other Biological Fluids
• Detection Range: 0.312-20ng/mL
• Sensitivity: The minimum detectable dose of this kit is typically less than 0.121ng/mL
• Precision: Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Stathmin 2 (STMN2) were tested 20 times on one plate, respectively.; Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Stathmin 2 (STMN2) were tested on 3 different plates, 8 replicates in each plate.; CV(%) = SD/meanX100; Intra-Assay: CV<10%; Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition.; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for STMN2 elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Stathmin 2 (STMN2). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Stathmin 2 (STMN2). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Stathmin 2 (STMN2), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Stathmin 2 (STMN2) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 51704330
• UniProt Accession #: Q93045
• NCBI Official Full Name: Stathmin-2
• UniProt Protein Name: Stathmin-2
• Protein Family: Stathmin
• UniProt Gene Name: STMN2
• UniProt Synonym Gene Names: SCG10; SCGN10; Protein SCG10
• UniProt Entry Name: STMN2_HUMAN

Uniprot Description

STMN2: a cytoskeletal protein that may play a role in neuronal differentiation, and in modulating membrane interaction with the cytoskeleton during neurite outgrowth. Associated with punctate structures in the perinuclear cytoplasm, axons, and growth cones of developing neurons. Neuron specific.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 8q21.13

Cellular Component: Golgi apparatus; neuron projection; growth cone; cell soma; membrane; perinuclear region of cytoplasm; axon; lamellipodium; cytoplasm; vesicle; endosome

Molecular Function: tubulin binding; protein binding; calcium-dependent protein binding

Biological Process: positive regulation of microtubule depolymerization; negative regulation of microtubule depolymerization; negative regulation of microtubule polymerization