Mouse CD14 Monoclonal Antibody | anti-CD14 antibody
anti-human CD14-Purified Preservative Free
Reactivity
Human, Monkey
Applications
Flow Cytometry, Functional Assay
Purity
Purified Preservative Free
Synonyms
CD14; Monoclonal Antibody; anti-human CD14-Purified Preservative Free; Monoclonal anti-human CD14; anti-CD14 antibody
Host
Mouse
Reactivity
Human, Monkey
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
UCHM1
Purity/Purification
Purified Preservative Free
Form/Format
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl
Concentration
1.0 mg/ml (varies by lot)
Sequence Length
373
Applicable Applications for anti-CD14 antibody
Flow Cytometry (FC/FACS)
Performance
Five x 105 ficoll prepared human peripheral blood mononuclear cells were washed and pre incubated 5 minutes with 20mul human IgG at 250 mug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80 mul of anti-CD14 antibody at 10 mug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. A net 4.8% sub population of the cells stained positive with a mean shift of 1.6 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration.
Production
Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 mum filtered and vialed under aseptic conditions.
Buffer
50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen
Human thymocytes/Sezary T cells
Preparation and Storage
Store at 2 - 5 degree C. Open under aseptic conditions. Freeze/Thawing is not recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Product should retain activity for at least 12 months after shipping date when stored as recommended.
Related Product Information for anti-CD14 antibody
Information: Human CD14 is strongly expressed on myelomonocyte lineage cells including monocytes, macrophages, and a subset of granulocytes. CD14 is a high affinity receptor for complexes of lipopolysaccharide and lipopolysaccharide binding protein. A soluble form of CD14 also binds to LPS and LBP. Antibody UCHM1 recognizes a membrane bond CD14 molecule of about 53 kd, and blocks LPS induced TNFUCHM1 will also immunoprecipitate a soluble form of CD14.
References
1) N. Hogg, et al, (1984) Immunology 53: 753-767. 2) M.O. Labeta, et al, (1993) Eur J Immunol 23: 2144-2151. 3) Leukocyte Typing IV (W. Knapp, et al, eds.) Oxford University Press, Oxford, (1989) "Reference Antibody". 4) Leukocyte Typing V (S.F. Schlossman, et al, eds.) Oxford University Press, Oxford, (1995) p. 778-788. 5) S.D. Wright, (1995) J Immunol 155: 6-8.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
39,667 Da
NCBI Official Full Name
CD14
NCBI Official Synonym Full Names
CD14 molecule<
NCBI Official Symbol
CD14
NCBI Protein Information
monocyte differentiation antigen CD14; CD14 antigen; myeloid cell-specific leucine-rich glycoprotein
UniProt Protein Name
Monocyte differentiation antigen CD14
Protein Family
UniProt Gene Name
CD14
UniProt Entry Name
CD14_BOVIN
Uniprot Description
Coreceptor for bacterial lipopolysaccharide. In concert with LBP, binds to monomeric lipopolysaccharide and delivers it to the LY96/TLR4 complex, thereby mediating the innate immune response to bacterial lipopolysaccharide (LPS). Acts via MyD88, TIRAP and TRAF6, leading to NF-kappa-B activation, cytokine secretion and the inflammatory response. Acts as a coreceptor for TLR2:TLR6 heterodimer in response to diacylated lipopeptides and for TLR2:TLR1 heterodimer in response to triacylated lipopeptides, these clusters trigger signaling from the cell surface and subsequently are targeted to the Golgi in a lipid-raft dependent pathway. Binds electronegative LDL (LDL-) and mediates the cytokine release induced by LDL- ().
Research Articles on CD14
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Product Notes
The CD14 cd14 (Catalog #AAA666415) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD14-Purified Preservative Free reacts with Human, Monkey and may cross-react with other species as described in the data sheet. AAA Biotech's CD14 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD14, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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