Mouse anti-Human CD134 Ligand Monoclonal Antibody | anti-TNFSF4 antibody
CD134 Ligand (CD134L, OX40L, CD252) (FITC)
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Affinity PurifiedPurified by Protein A affinity chromatography.
Synonyms
CD134 Ligand; Monoclonal Antibody; CD134 Ligand (CD134L; OX40L; CD252) (FITC); Anti -CD134 Ligand (CD134L; anti-TNFSF4 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Clone Number
7H146
Specificity
Binds to CD134L on the cell surface of HUVEC in Flow cytometry, and blocks binding of recombinant CD134-muIg.
Purity/Purification
Affinity Purified
Purified by Protein A affinity chromatography.
Purified by Protein A affinity chromatography.
Form/Format
Supplied as a liquid in 50mM sodium phosphate pH 7.5, 100mM potassium chloride, 150mM sodium chloride, 0.2% BSA, 0.04% sodium azide, 5% glycerol. Labeled with FITC. Molar Ratio: 8.1 FITC /IgG.
Applicable Applications for anti-TNFSF4 antibody
ELISA (EL/EIA), Flow Cytometry (FC/FACS)
Application Notes
Suitable for use in Flow Cytometry, ELISA and blocking studies.
Dilution: Flow Cytometry: 1:50 (or 1.6ul neat/5x10e5 cells/test)
Blocking: Blocks binding of Recombinant CD134 to CD134L on HUVEC.
Flow Cytometry Protocol: 1. Cultured human umbilical cord vein endothelial cells (HUVEC) were harvested by trypsinization. 5x10e5 cells per tube were washed and incubated 45 minutes on ice with 80ul of C2515-40C at a 1:50 dilution (10ug/ml).
2. Washed three times, fixed and analyzed on a BD FACS Calibur A. Binding was 87% blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml C2515-40A.
Dilution: Flow Cytometry: 1:50 (or 1.6ul neat/5x10e5 cells/test)
Blocking: Blocks binding of Recombinant CD134 to CD134L on HUVEC.
Flow Cytometry Protocol: 1. Cultured human umbilical cord vein endothelial cells (HUVEC) were harvested by trypsinization. 5x10e5 cells per tube were washed and incubated 45 minutes on ice with 80ul of C2515-40C at a 1:50 dilution (10ug/ml).
2. Washed three times, fixed and analyzed on a BD FACS Calibur A. Binding was 87% blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml C2515-40A.
Immunogen
Human HUVEC and Recombinant soluble human CD134L
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for anti-TNFSF4 antibody
Human CD134L (OX-40 Ligand) is a type II membrane protein with homology to TNF which is expressed on activated B cells and activated endothelial cells. CD134L binds to CD134 present on activated T cells, providing a costimulatory signal (1). This pathway seems to be more effective for costimulation of CD4+ helper T cells than for CD8+ effector T cells (4). Blockade of this interaction in mouse abrogated immunological effects in several models of immflamation and rejection (5,6,7,8).
Product Categories/Family for anti-TNFSF4 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Official Full Name
CD134(OX40)-ligand
NCBI Official Symbol
TNFSF4
NCBI Protein Information
tumor necrosis factor ligand superfamily member 4; CD134(OX40)-ligand
Protein Family
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Product Notes
The TNFSF4 (Catalog #AAA600114) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD134 Ligand (CD134L, OX40L, CD252) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD134 Ligand can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Flow Cytometry (FC/FACS). Suitable for use in Flow Cytometry, ELISA and blocking studies. Dilution: Flow Cytometry: 1:50 (or 1.6ul neat/5x10e5 cells/test) Blocking: Blocks binding of Recombinant CD134 to CD134L on HUVEC. Flow Cytometry Protocol: 1. Cultured human umbilical cord vein endothelial cells (HUVEC) were harvested by trypsinization. 5x10e5 cells per tube were washed and incubated 45 minutes on ice with 80ul of C2515-40C at a 1:50 dilution (10ug/ml). 2. Washed three times, fixed and analyzed on a BD FACS Calibur A. Binding was 87% blocked when cells were pre-incubated 10 minutes with 20ul of 0.5mg/ml C2515-40A. Researchers should empirically determine the suitability of the TNFSF4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD134 Ligand, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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