Mouse anti-Human IgA Monoclonal Antibody | anti-IgA antibody

anti-human IgA-Biotin

Cat. Num. AAA666334

• Reactivity: Human
• Applications: Flow Cytometry, Functional Assay, ELISA
• Synonyms: IgA; Monoclonal Antibody; anti-human IgA-Biotin; Monoclonal anti-human IgA /Biotin; anti-IgA antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1k
• Clone Number: Hisa43
• Form/Format: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 131

• Applicable Applications for anti-IgA antibody: Flow Cytometry (FC/FACS), ELISA (EIA)
• Performance: Five x 105 ficoll prepared human peripheral white blood cells were pre incubated 5 minutes with 20 ul of 250 ug/ml human Ig after which they were incubated 45 minutes on ice with 80 ul of anti-human IgA/Biotin at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS. A net 8.6% sub population of the cells stained positive with a mean shift of 1.22 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-IgA antibody.
• Conjugation: Biotin
• Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
• Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
• Immunogen: Human salivary proteins
• Preparation and Storage: Store at 2 - 5 degree C. Freeze/thawing not recommended.; Product should retain activity for at least 12 months after shipping date when stored as recommended.

Testing Data

Related Product Information for anti-IgA antibody

Information: Human immunoglobulins are glycoproteins composed of two disulfide-bonded heavy (H) chain subunits, each of which is linked by interchain disulfide bonds to a light (L) chain forming a tetramolecular complex. There are five classes of immunoglobulins, designated IgG, IgA, IgM, IgD and IgE, which are defined by differences in the constant region of H chains. L chains are divided into kappa or lambda classifications based on structural antigenic differences. All classes of immunoglobulins have been found on the cell surface of B lymphocytes where they function as antigen receptors to elicit antigen-dependent proliferation and secretion of antigen specific soluble circulating antibodies. Antibody Hisa43 recognizes cell surface expressed IgA.

References

Basic and Clinical Immunology, Seventh edition (D. P. Sites & A. I. Terr, eds.) Appleton & Lange., Norwalk, CT (1991). 2. J. Biewenga, et al, (1986) Mol Immunol 23: 761-767.

NCBI and Uniprot Product Information

• NCBI GI #: 2632187
• NCBI Official Full Name: IgA, partial
• Protein Family: IgA receptor