Flow Cytometry (FC/FACS)
(Staining of C57BL/6 bone marrow cells with CD45R antibody (FITC) and 0.125 ug of Rat IgG2b kappa Isotype Control (biotin) (left) or 0.125 ug of CD117 antibody (c-Kit) (biotin) (right) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Rat anti-Mouse CD117 Monoclonal Antibody | anti-CD117 antibody
CD117 antibody (biotin)
Reactivity
Mouse
Applications
Flow Cytometry, Functional Assay
Synonyms
CD117; Monoclonal Antibody; CD117 antibody (biotin); Rat monoclonal CD117 antibody (biotin); Monoclonal CD117 antibody; Anti-CD117 antibody; Steel Factor Receptor antibody; CD 117; CD-117 antibody; CD 117 antibody; cKit antibody; Proto-oncogene c-Kit antibody; Tyrosine-protein kinase Kit antibody; SCFR antibody; CD-117; Mast/stem cell growth factor receptor antibody; anti-CD117 antibody
Host
Rat
Reactivity
Mouse
Clonality
Monoclonal
Isotype
IgG2b kappa
Clone Number
2B8
Specificity
Mouse
Form/Format
Supplied in aqueous buffer with 0.09% sodium azide, may contain carrier protein/stabilizer.
Concentration
500 ug/ml (varies by lot)
Applicable Applications for anti-CD117 antibody
Flow Cytometry (FC/FACS)
Application Notes
FC/FACS: = 0.25 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Conjugate
Biotin
Preparation and Storage
Store at 2-8 degree C. Do not freeze. Protect from light.
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 bone marrow cells with CD45R antibody (FITC) and 0.125 ug of Rat IgG2b kappa Isotype Control (biotin) (left) or 0.125 ug of CD117 antibody (c-Kit) (biotin) (right) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS)
(Staining of C57BL/6 bone marrow cells with CD45R antibody (FITC) and 0.125 ug of Rat IgG2b kappa Isotype Control (biotin) (left) or 0.125 ug of CD117 antibody (c-Kit) (biotin) (right) followed by Streptavidin (PE). Total viable cells were used for analysis.)
Related Product Information for anti-CD117 antibody
The 2B8 monoclonal antibody reacts with mouse CD117, also known as c-Kit receptor, Steel factor receptor and stem cell factor receptor. A member of the tyrosine kinase receptor family, this 145 kDa molecule is expressed by a majority of hematopoietic progenitor cells characterized in the mouse bone marrow as a small subset of cells positive for Sca-1 and Thy1 and negative for lineage markers. The interaction of the mouse c-kit receptor and steel factor promotes the proliferation and differentiation of hematopoietic progenitor cells. CD117 is also expressed by mast cells and plays a role in signaling and activation of these cells.
Product Categories/Family for anti-CD117 antibody
Similar Products
Product Notes
The CD117 (Catalog #AAA5309435) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD117 antibody (biotin) reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's CD117 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). FC/FACS: = 0.25 ug/test A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 105 to 108 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD117 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD117, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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