Coagulation Factor X (F10) Recombinant Protein | F10 recombinant protein

Recombinant Coagulation Factor X (F10)

Cat. Num. AAA2097550

• Gene Names: F10; FX; FXA
• Reactivity: Human
• Applications: SDS-Page, Western Blot
• Purity: > 90%
• Synonyms: Coagulation Factor X (F10); Recombinant Coagulation Factor X (F10); FX; FXA; Stuart Prower Factor; Thrombokinase; F10 recombinant protein

• Host: E Coli
• Reactivity: Human
• Purity/Purification: > 90%
• Form/Format: Freeze-dried powder
• Sequence Length: 488

• Applicable Applications for F10 recombinant protein: Positive Control, Immunogen, SDS-PAGE, Western Blot (WB)
• Expression System: Prokaryotic expression
• Tag: N-terminal His and GST Tag
• Buffer Formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Endotoxin Level: <1.0EU per 1ug (determined by the LAL method)
• Fragment: Ile235~Lys488
• Subcellular Location: Secreted
• Isoelectric Point: 8.5

Sequence Information

SDS-Page

Product Categories/Family for F10 recombinant protein

Signal transduction; Hematology

NCBI and Uniprot Product Information

• NCBI GI #: 4503625
• NCBI GeneID: 2159
• NCBI Accession #: NP_000495.1
• NCBI GenBank Nucleotide #: NM_000504.3
• UniProt Accession #: P00742; Q14340
• Molecular Weight: Predicted Molecular Weight: 58.5kDa; Accurate Molecular Weight: 59kDa
• NCBI Official Full Name: coagulation factor X isoform 1 preproprotein
• NCBI Official Synonym Full Names: coagulation factor X
• NCBI Official Symbol: F10
• NCBI Official Synonym Symbols: FX; FXA
• NCBI Protein Information: coagulation factor X
• UniProt Protein Name: Coagulation factor X
• Protein Family: Coagulation factor
• UniProt Gene Name: F10

NCBI Description

This gene encodes the vitamin K-dependent coagulation factor X of the blood coagulation cascade. This factor undergoes multiple processing steps before its preproprotein is converted to a mature two-chain form by the excision of the tripeptide RKR. Two chains of the factor are held together by 1 or more disulfide bonds; the light chain contains 2 EGF-like domains, while the heavy chain contains the catalytic domain which is structurally homologous to those of the other hemostatic serine proteases. The mature factor is activated by the cleavage of the activation peptide by factor IXa (in the intrisic pathway), or by factor VIIa (in the extrinsic pathway). The activated factor then converts prothrombin to thrombin in the presence of factor Va, Ca+2, and phospholipid during blood clotting. Mutations of this gene result in factor X deficiency, a hemorrhagic condition of variable severity. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar proteolytic processing to generate mature polypeptides. [provided by RefSeq, Aug 2015]

Uniprot Description

F10: Factor Xa is a vitamin K-dependent glycoprotein that converts prothrombin to thrombin in the presence of factor Va, calcium and phospholipid during blood clotting. Defects in F10 are the cause of factor X deficiency (FA10D). A hemorrhagic disease with variable presentation. Affected individuals can manifest prolonged nasal and mucosal hemorrhage, menorrhagia, hematuria, and occasionally hemarthrosis. Some patients do not have clinical bleeding diathesis. Belongs to the peptidase S1 family.

Protein type: EC 3.4.21.6; Lipid-binding; Motility/polarity/chemotaxis; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 13q34

Cellular Component: endoplasmic reticulum lumen; extracellular region; Golgi lumen; plasma membrane

Molecular Function: calcium ion binding; phospholipid binding; protein binding; serine-type endopeptidase activity

Biological Process: blood coagulation; blood coagulation, extrinsic pathway; blood coagulation, intrinsic pathway; ER to Golgi vesicle-mediated transport; peptidyl-glutamic acid carboxylation; positive regulation of cell migration; positive regulation of protein kinase B signaling; signal peptide processing

Disease: Factor X Deficiency