Human Nitric Oxide Synthase Trafficker (NOSTRIN) ELISA Kit | NOSTRIN elisa kit

Human Nitric Oxide Synthase Trafficker (NOSTRIN) ELISA Kit

Cat. Num. AAA2022361

• Gene Names: NOSTRIN; DaIP2
• Reactivity: Human
• Synonyms: Nitric Oxide Synthase Trafficker (NOSTRIN); Human Nitric Oxide Synthase Trafficker (NOSTRIN) ELISA Kit; BM247 homolog; Nitric oxide synthase traffic inducer; eNOS-trafficking inducer; NOSTRIN elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of Nitric Oxide Synthase Trafficker (NOSTRIN).; No significant cross-reactivity or interference between Nitric Oxide Synthase Trafficker (NOSTRIN) and analogues was observed.
• Sequence Length: 563

• Assay Type: Double-antibody Sandwich
• Samples: Tissue homogenates, Cell lysates and Other Biological Fluids
• Detection Range: 0.156-10ng/mL
• Sensitivity: < 0.058ng/mL
• Application: Enzyme-linked immunosorbent assay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Nitric Oxide Synthase Trafficker (NOSTRIN) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Nitric Oxide Synthase Trafficker (NOSTRIN) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for NOSTRIN elisa kit

The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Nitric Oxide Synthase Trafficker (NOSTRIN). Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody specific to Nitric Oxide Synthase Trafficker (NOSTRIN). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Nitric Oxide Synthase Trafficker (NOSTRIN), biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Nitric Oxide Synthase Trafficker (NOSTRIN) in the samples is then determined by comparing the O.D. of the samples to the standard curve.

Product Categories/Family for NOSTRIN elisa kit

Metabolic pathway; Infection immunity; Cardiovascular biology; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 284172483
• NCBI GeneID: 115677
• NCBI Accession #: NP_001165103.1
• NCBI GenBank Nucleotide #: NM_001171632.1
• UniProt Accession #: Q8IVI9; Q27HG3; Q53S62; Q96CJ9; A8K2I9; B3KSF5; E7EPT9
• Molecular Weight: 64,364 Da
• NCBI Official Full Name: nostrin isoform 3
• NCBI Official Synonym Full Names: nitric oxide synthase trafficking
• NCBI Official Symbol: NOSTRIN
• NCBI Official Synonym Symbols: DaIP2
• NCBI Protein Information: nostrin
• UniProt Protein Name: Nostrin
• Protein Family: Nostrin
• UniProt Gene Name: NOSTRIN
• UniProt Entry Name: NOSTN_HUMAN

NCBI Description

Nitric oxide (NO) is a potent mediator in biologic processes such as neurotransmission, inflammatory response, and vascular homeostasis. NOSTRIN binds the enzyme responsible for NO production, endothelial NO synthase (ENOS; MIM 163729), and triggers the translocation of ENOS from the plasma membrane to vesicle-like subcellular structures, thereby attenuating ENOS-dependent NO production.[supplied by OMIM, Apr 2004]

Uniprot Description

NOSTRIN: Multivalent adapter protein which may decrease NOS3 activity by inducing its translocation away from the plasma membrane. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Adaptor/scaffold

Chromosomal Location of Human Ortholog: 2q31.1

Cellular Component: cytoskeleton; plasma membrane; nucleus

Molecular Function: protein binding; DNA binding

Biological Process: endocytosis; regulation of nitric-oxide synthase activity; negative regulation of transcription, DNA-dependent; signal transduction; nitric oxide metabolic process