Rabbit anti-Human Caspase 5 Monoclonal Antibody | anti-CASP5 antibody

Caspase 5 Antibody

Cat. Num. AAA9435910

• Gene Names: CASP5; ICH-3; ICEREL-III; ICE(rel)III
• Reactivity: Human
• Applications: Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation
• Purity: ProA affinity purified
• Synonyms: Caspase 5; Monoclonal Antibody; Caspase 5 Antibody; Apoptosis related cysteine protease antibody; CASP-5 antibody; CASP5 antibody; CASP5_HUMAN antibody; Caspase-5 subunit p10 antibody; ICE (rel)-III antibody; ICERELIII antibody; ICH 3 antibody; ICH 3 protease antibody; Protease ICH-3 antibody; Protease TY antibody; TY antibody; TY protease antibody; anti-CASP5 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Clone Number: SD203-2
• Purity/Purification: ProA affinity purified
• Form/Format: 1*TBS (pH7.4), 1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-CASP5 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC), Immunoprecipitation (IP)
• Application Notes: WB: 1:500-1:1000; IHC: 1:50-1:200; ICC: 1:50-1:200
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant protein
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Caspase 5 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining Caspase 5 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Caspase 5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining Caspase 5 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-CASP5 antibody

Caspases are cysteine proteases which play important roles in the activation of cytokines and in apoptosis. The ICE subfamily of caspases comprises peptides closely related to caspase-1, which promotes maturation of interleukin 1beta (IL-1beta ) and interleukin-18 (IL-18) by proteolytic cleavage of precursor forms to generate biologically active peptides. Both caspase-4 and caspase-5 are members of the caspase-1 subfamily, and are more closely related to each other than to other homologues. Caspase-5 (also designated ICErel-III, TY, ICH-3 and caspase-12 in mouse), can cleave its own precursor, an activity that requires the cysteine 245 residue. Frameshift mutations in caspase-5 have been identified in MMP tumors of the endometrium, colon, and stomach, indicating the caspase-5 may be a new target gene in the microsatellite mutator pathway for cancer. The human caspase 5 gene maps to chromosome 11q22.2-q22.3 and encodes a protein whose expression is barely detectable in most tissues except brain, with highest expression levels being found in lung, liver and skeletal muscle.

Product Categories/Family for anti-CASP5 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 209870071
• NCBI GeneID: 838
• NCBI Accession #: NP_001129581.1
• NCBI GenBank Nucleotide #: NM_001136109.1
• UniProt Accession #: P51878; Q0QVY7; Q0QVY8; Q0QVZ0; Q0QVZ1; Q0QVZ2; Q14DD6; Q1HBJ3; Q6DJV7; B4DKP5
• Molecular Weight: 49,736 Da
• NCBI Official Full Name: caspase-5 isoform b
• NCBI Official Synonym Full Names: caspase 5, apoptosis-related cysteine peptidase
• NCBI Official Symbol: CASP5
• NCBI Official Synonym Symbols: ICH-3; ICEREL-III; ICE(rel)III
• NCBI Protein Information: caspase-5; CASP-5; TY protease; protease TY; ICE(rel)-III; protease ICH-3; caspase 5, apoptosis-related cysteine protease
• UniProt Protein Name: Caspase-5
• Protein Family: Caspase
• UniProt Gene Name: CASP5
• UniProt Synonym Gene Names: ICH3; CASP-5
• UniProt Entry Name: CASP5_HUMAN

NCBI Description

This gene encodes a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. Overexpression of the active form of this enzyme induces apoptosis in fibroblasts. Max, a central component of the Myc/Max/Mad transcription regulation network important for cell growth, differentiation, and apoptosis, is cleaved by this protein; this process requires Fas-mediated dephosphorylation of Max. The expression of this gene is regulated by interferon-gamma and lipopolysaccharide. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Aug 2010]

Uniprot Description

CASP5: Mediator of programmed cell death (apoptosis). Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 20 kDa (p20) and a 10 kDa (p10) subunits. Up-regulated by bacterial lipopolysaccharides (LPS). Expressed in barely detectable amounts in most tissues except brain, highest levels being found in lung, liver and skeletal muscle. Belongs to the peptidase C14A family. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.4.22.58; Protease

Chromosomal Location of Human Ortholog: 11q22.2-q22.3

Cellular Component: neuron projection; cell soma; cytoplasm

Molecular Function: cysteine-type endopeptidase activity; cysteine-type peptidase activity

Biological Process: regulation of apoptosis; substantia nigra development; apoptosis; regulation of inflammatory response; germ cell programmed cell death; positive regulation of interleukin-1 beta secretion; proteolysis