Mouse Interleukin 34 (IL34) CLIA Kit | IL34 clia kit

Mouse Interleukin 34 (IL34) CLIA Kit

Cat. Num. AAA2000758

• Gene Names: IL34; IL-34; C16orf77
• Reactivity: Mouse
• Synonyms: Interleukin 34 (IL34); Mouse Interleukin 34 (IL34) CLIA Kit; C16orf77; IL34 clia kit

• Reactivity: Mouse
• Specificity: This assay has high sensitivity and excellent specificity for detection of Interleukin 34 (IL34).; No significant cross-reactivity or interference between Interleukin 34 (IL34) and analogues was observed.
• Sequence Length: 242

• Assay Type: Double-antibody Sandwich
• Samples: Serum, Plasma, Tissue homogenates, Cell culture supernates and Other Biological Fluids
• Detection Range: 1.37-1,000pg/mL
• Sensitivity: < 0.53pg/mL
• Application: Chemiluminescent immunoassay for Antigen Detection.
• Intra-assay Precision (Precision within an assay): 3 samples with low, middle and high level Interleukin 34 (IL34) were tested 20 times on one plate, respectively.
• Inter-assay Precision (Precision between assays): 3 samples with low, middle and high level Interleukin 34 (IL34) were tested on 3 different plates, 8 replicates in each plate.
• CV(%) =: SD/meanX100
• Intra-Assay: CV<10%
• Inter-Assay: CV<12%
• Preparation and Storage: The stability of kit is determined by the loss rate of activity. The loss rate of this kit is less than 5% within the expiration date under appropriate storage condition. ; To minimize extra influence on the performance, operation procedures and lab conditions, especially room temperature, air humidity, incubator temperature should be strictly controlled. It is also strongly suggested that the whole assay is performed by the same operator from the beginning to the end.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for IL34 clia kit

The microplate provided in this kit has been pre-coated with an antibody specific to Interleukin 34 (IL34). Standards or samples are then added to the appropriate microplate wells with a biotin-conjugated antibody specific to Interleukin 34 (IL34). Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then the mixture of substrate A and B is added to generate glow light emission kinetics. Upon plate development, the intensity of the emitted light is proportional to the Interleukin 34 (IL34) level in the sample or standard.;

Product Categories/Family for IL34 clia kit

Cytokine; Infection immunity

NCBI and Uniprot Product Information

• NCBI GI #: 289666752
• NCBI GeneID: 146433
• NCBI Accession #: NP_001166243.1
• NCBI GenBank Nucleotide #: NM_001172772.1
• UniProt Accession #: Q6ZMJ4; Q8N6L2; B2RC28; B2Z4A8; B2ZC70
• Molecular Weight: 27,354 Da
• NCBI Official Full Name: interleukin-34 isoform 1
• NCBI Official Synonym Full Names: interleukin 34
• NCBI Official Symbol: IL34
• NCBI Official Synonym Symbols: IL-34; C16orf77
• NCBI Protein Information: interleukin-34
• UniProt Protein Name: Interleukin-34
• Protein Family: Interleukin
• UniProt Gene Name: IL34
• UniProt Synonym Gene Names: C16orf77; IL-34
• UniProt Entry Name: IL34_HUMAN

NCBI Description

Interleukin-34 is a cytokine that promotes the differentiation and viability of monocytes and macrophages through the colony-stimulating factor-1 receptor (CSF1R; MIM 164770) (Lin et al., 2008 [PubMed 18467591]).[supplied by OMIM, May 2008]

Uniprot Description

IL34: Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1. Belongs to the IL-34 family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Cytokine; Cell cycle regulation; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 16q22.1

Cellular Component: extracellular space

Molecular Function: growth factor activity; cytokine activity; macrophage colony stimulating factor receptor binding

Biological Process: positive regulation of cell proliferation; innate immune response; positive regulation of protein amino acid phosphorylation; inflammatory response