Mouse anti-Human Carboxypeptidase B1 Monoclonal Antibody | anti-CPB1 antibody

Anti-Carboxypeptidase B1 Antibody, Mouse Monoclonal

Cat. Num. AAA8103009

• Gene Names: CPB1; CPB; PASP; PCPB
• Reactivity: Human
• Applications: Immunohistochemistry
• Purity: Protein A
• Synonyms: Carboxypeptidase B1; Monoclonal Antibody; Anti-Carboxypeptidase B1 Antibody; Mouse Monoclonal; Carboxypeptidase B1/CPB1 Antibody; Mouse MAb; carboxypeptidase B1 (tissue); Anti-CPB Antibody; Anti-PASP Antibody; Anti-PCPB Antibody; anti-CPB1 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1
• Clone Number: 07
• Specificity: Human Carboxypeptidase B1/CPB1.; No cross-reactivity in ELISA with Human CPB1, Mouse CPB1, Human CPA1, Human CPA2, Human CPE
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CPB1 antibody: Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: IHC-P: 1:1000-1:4000
• Immunogen: Recombinant Human Carboxypeptidase B1/CPB1 protein
• Conjugation: Unconjugated
• Preparation: This antibody was produced from a hybridoma resulting from the fusion of a mouse myeloma with B cells obtained from a mouse immunized with purified, recombinant Human Carboxypeptidase B1/CPB1 (rh Carboxypeptidase B1/CPB1). The IgG fraction of the cell culture supernatant was purified by Protein A affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CPB1 in human pancreas with mouse monoclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Related Product Information for anti-CPB1 antibody

Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 37-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

References

Yamamoto, K.K. et al., 1992, J. Biol. Chem. 267: 2575-2581. Pezzilli, R. et al., 1994, Digestion. 55: 73-77. Barbosa Pereira, P.J. et al., 2002, J. Mol. Biol. 321: 537-547.

NCBI and Uniprot Product Information

• NCBI GI #: 54607080
• NCBI GeneID: 1360
• NCBI Accession #: NP_001862.2
• NCBI GenBank Nucleotide #: NM_001871.2
• UniProt Accession #: P15086; O60834; Q53XJ0; Q96BQ8
• Molecular Weight: 47,368 Da
• NCBI Official Full Name: carboxypeptidase B preproprotein
• NCBI Official Synonym Full Names: carboxypeptidase B1
• NCBI Official Symbol: CPB1
• NCBI Official Synonym Symbols: CPB; PASP; PCPB
• NCBI Protein Information: carboxypeptidase B
• UniProt Protein Name: Carboxypeptidase B
• UniProt Gene Name: CPB1
• UniProt Synonym Gene Names: CPB; PCPB; PASP
• UniProt Entry Name: CBPB1_HUMAN

NCBI Description

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]