Rabbit anti-Human ANK1 Polyclonal Antibody | anti-ANK1 antibody

ANK1 (Ankyrin-1, Ankyrin 1, ANK-1, Ankyrin-R, Erythrocyte Ankyrin, ANK) (HRP)

Cat. Num. AAA6369851

• Gene Names: ANK1; ANK; SPH1; SPH2
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: ANK1; Polyclonal Antibody; ANK1 (Ankyrin-1; Ankyrin 1; ANK-1; Ankyrin-R; Erythrocyte Ankyrin; ANK) (HRP); anti-ANK1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human ANK1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).
• Sequence Length: 1084

• Applicable Applications for anti-ANK1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human ANK1, aa1-155 (AAH30957.1).
• Immunogen Sequence: MWTFVTQLLVTLVLLSFFLVSCQNVMHIVRGSLCFVLKHIHQELDKELGESEDLSDDEETISTRVVRRRVFLKGNEFQNIPGEQVTEEQFTDEQGNIVTKKIIRKVVRQIDLSSADAAQEHEEVELRGSGLQPDLIEGRKGAQIVKRASLKRGKQ
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of ANK1 expression in transfected 293T cell line by ANK1 polyclonal antibody. Lane 1: ANK1 transfected lysate (17.7kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-ANK1 antibody

Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin actin cytoskeleton and therefore play a role in cell motility, proliferation and intercellular contact. Different isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains. Ankyrin 1, the prototype of this family, was first discovered in erythrocytes but has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated with hereditary spherocytosis. Different isoforms of ankyrin 1 have been described. Alternative polyadenylation accounting for the different sized erythrocytic ankyrin 1 mRNAs, has also been reported.

Product Categories/Family for anti-ANK1 antibody

Antibodies; Neuroscience

NCBI and Uniprot Product Information

• NCBI GI #: 21410935
• NCBI GeneID: 286
• NCBI Official Full Name: Homo sapiens ankyrin 1, erythrocytic, mRNA
• NCBI Official Synonym Full Names: ankyrin 1
• NCBI Official Symbol: ANK1
• NCBI Official Synonym Symbols: ANK; SPH1; SPH2
• NCBI Protein Information: ankyrin-1
• Protein Family: Ankyrin

NCBI Description

Ankyrins are a family of proteins that link the integral membrane proteins to the underlying spectrin-actin cytoskeleton and play key roles in activities such as cell motility, activation, proliferation, contact and the maintenance of specialized membrane domains. Multiple isoforms of ankyrin with different affinities for various target proteins are expressed in a tissue-specific, developmentally regulated manner. Most ankyrins are typically composed of three structural domains: an amino-terminal domain containing multiple ankyrin repeats; a central region with a highly conserved spectrin binding domain; and a carboxy-terminal regulatory domain which is the least conserved and subject to variation. Ankyrin 1, the prototype of this family, was first discovered in the erythrocytes, but since has also been found in brain and muscles. Mutations in erythrocytic ankyrin 1 have been associated in approximately half of all patients with hereditary spherocytosis. Complex patterns of alternative splicing in the regulatory domain, giving rise to different isoforms of ankyrin 1 have been described. Truncated muscle-specific isoforms of ankyrin 1 resulting from usage of an alternate promoter have also been identified. [provided by RefSeq, Dec 2008]