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SDS-PAGE

Carboxypeptidase B1 / CPB1 Recombinant Protein | CPB1 recombinant protein

Recombinant Human Carboxypeptidase B1 / CPB1 Protein (His tag)

Gene Names
CPB1; CPB; PASP; PCPB
Purity
> 98 % as determined by SDS-PAGE
Synonyms
Carboxypeptidase B1 / CPB1; Recombinant Human Carboxypeptidase B1 / CPB1 Protein (His tag); CPB1; DKFZp779K1333; PASP; PCPB; CPB1 recombinant protein
Ordering
For Research Use Only!
Host
Human Cells
Purity/Purification
> 98 % as determined by SDS-PAGE
Form/Format
Lyophilized from sterile 25mM MES, 0.1 M NaCl, pH 6.5
Sequence Length
417
Application Notes
The recombinant human CPB1 consists of 413 amino acids and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 45 kDa band.
Predicted N Terminal
His 16
Endotoxin
< 1.0 EU per mug of the protein as determined by the LAL method
Preparation and Storage
Samples are stable for up to twelve months from date of receipt at -70 degree C

SDS-PAGE

SDS-PAGE
Related Product Information for CPB1 recombinant protein
Background: Human Carboxypeptidase B1, also well known as pancreatic procarboxypeptidase B (PCPB), is a highly pancreas -specific protein (PASP), and has been identified previously as a serum marker for acute pancreatitis and pancreatic graft rejection. As the prototype for those human exopeptidases that cleave off basic C-terminal residues, CPB1 specifically cleaves the C-terminal Arg and Lys residues with a preference for Arg. The B1 and B2 forms of procarboxypeptidase B differ from each other mainly in isoelectric point.The deduced amino acid sequence of PCPB predicts a 416-amino acid preproenzyme consisting of a 15-aa signal peptide, a 95-aa activation peptide and a 307-aa mature chain. The secreted PCPB zymogen is converted to enzymatically active CPB1 by limited proteolysis by trypsin.

Description: A DNA sequence encoding the human CPB1 (NP_001862.2) (Met 1-Tyr 417) was expressed with a C-terminal polyhistidine tag.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,368 Da
NCBI Official Full Name
carboxypeptidase B preproprotein
NCBI Official Synonym Full Names
carboxypeptidase B1
NCBI Official Symbol
CPB1
NCBI Official Synonym Symbols
CPB; PASP; PCPB
NCBI Protein Information
carboxypeptidase B
UniProt Protein Name
Carboxypeptidase B
UniProt Gene Name
CPB1
UniProt Synonym Gene Names
CPB; PCPB; PASP

NCBI Description

Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]

Uniprot Description

CPB1: Three different procarboxypeptidases A and two different procarboxypeptidases B have been isolated. The B1 and B2 forms differ from each other mainly in isoelectric point. Carboxypeptidase B1 is a highly tissue-specific protein and is a useful serum marker for acute pancreatitis and dysfunction of pancreatic transplants. It is not elevated in pancreatic carcinoma. [provided by RefSeq, Jul 2008]

Protein type: EC 3.4.17.2; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q24

Cellular Component: extracellular space

Molecular Function: carboxypeptidase activity; metallocarboxypeptidase activity

Research Articles on CPB1

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Product Notes

The CPB1 cpb1 (Catalog #AAA2546220) is a Recombinant Protein produced from Human Cells and is intended for research purposes only. The product is available for immediate purchase. The recombinant human CPB1 consists of 413 amino acids and has a predicted molecular mass of 47 kDa. In SDS-PAGE under reducing conditions, it migrates as an approximately 45 kDa band. Researchers should empirically determine the suitability of the CPB1 cpb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carboxypeptidase B1 / CPB1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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