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Immunohistochemistry (IHC) (Immunochemical staining of mouse CPA2 in mouse pancreas with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Rabbit anti-Mouse Carboxypeptidase A2 Monoclonal Antibody | anti-CPA2 antibody

Recombinant Anti-Carboxypeptidase A2 Antibody, Rabbit Monoclonal

Reactivity
Mouse
Applications
Immunohistochemistry
Purity
Protein A
Synonyms
Carboxypeptidase A2; Monoclonal Antibody; Recombinant Anti-Carboxypeptidase A2 Antibody; Rabbit Monoclonal; Carboxypeptidase A2/CPA2 Antibody; Rabbit MAb; carboxypeptidase A2; Anti-CPA2 Antibody; anti-CPA2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Mouse
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
133
Specificity
Mouse Carboxypeptidase A2/CPA2.
No cross-reactivity in ELISA with Mouse CPA1, Mouse CPB1
Purity/Purification
Protein A
Form/Format
Liquid; 0.2um filtered solution in PBS
Applicable Applications for anti-CPA2 antibody
Immunohistochemistry-Paraffin (IHC-P)
Application Notes
IHC-P: 1:100-1:500
Antibody Type
Recombinant Antibody
Immunogen
Recombinant Mouse Carboxypeptidase A2/CPA2 protein
Conjugation
Unconjugated
Preparation
This antibody was obtained from a rabbit immunized with purified, recombinant Mouse Carboxypeptidase A2/CPA2 (rM Carboxypeptidase A2/CPA2).
Preparation and Storage
This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of mouse CPA2 in mouse pancreas with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC) (Immunochemical staining of mouse CPA2 in mouse pancreas with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
Related Product Information for anti-CPA2 antibody
Carboxypeptidase A2 (CPA2) is a secreted pancreatic procarboxy -peptidase, and cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group. The hydrolytic action of CPA2 was identified with a preference towards long substrates with aromatic amino acids in their C-terminal end, particularly tryptophan. CPA2 comprises a signal peptide, a pro region and a mature chain, and can be activated after cleavage of the pro peptide. Three different forms of human pancreatic procarboxypeptidase A have been isolated, and the A1 and A2 forms are always secreted as monomeric proteins with different biochemical properties.
References
Catasus, L. et al., 1995. J. Biol. Chem. 270: 6651-6657. Aloy, P. et al., 1998, Biol. Chem. 379: 149-155. Laethem, RM. et al., 1996, Arch. Biochem. Biophys.332: 8-18.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
47,057 Da
NCBI Official Full Name
carboxypeptidase A2 preproprotein
NCBI Official Synonym Full Names
carboxypeptidase A2, pancreatic
NCBI Official Symbol
Cpa2
NCBI Protein Information
carboxypeptidase A2
UniProt Protein Name
Carboxypeptidase A2
Protein Family
UniProt Gene Name
Cpa2

NCBI Description

This gene encodes a member of the carboxypeptidase A family of zinc metalloproteases. The encoded preproprotein undergoes proteolytic processing that removes the N-terminal activation peptide to generate a functional enzyme. This gene is expressed by the pancreatic exocrine cells which secrete the enzyme during digestion. This gene is located in a cluster of carboxypeptidase genes on chromosome 6. [provided by RefSeq, Jul 2016]

Uniprot Description

CPA2: Three different forms of human pancreatic procarboxypeptidase A have been isolated. The encoded protein represents the A2 form, which is a monomeric protein with different biochemical properties from the A1 and A3 forms. The A2 form of pancreatic procarboxypeptidase acts on aromatic C-terminal residues and is a secreted protein. [provided by RefSeq, Dec 2008]

Protein type: EC 3.4.17.15; Protease; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 6|6 A3.3

Cellular Component: extracellular region; extracellular space

Molecular Function: carboxypeptidase activity; hydrolase activity; metal ion binding; metallocarboxypeptidase activity; metallopeptidase activity; peptidase activity; zinc ion binding

Biological Process: proteolysis

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Product Notes

The CPA2 cpa2 (Catalog #AAA8112061) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Recombinant Anti-Carboxypeptidase A2 Antibody, Rabbit Monoclonal reacts with Mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Carboxypeptidase A2 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry-Paraffin (IHC-P). IHC-P: 1:100-1:500. Researchers should empirically determine the suitability of the CPA2 cpa2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Carboxypeptidase A2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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