Mouse 40S ribosomal protein S14 ELISA Kit | Rps14 elisa kit

Mouse 40S ribosomal protein S14 ELISA Kit

Cat. Num. AAA762696

• Gene Names: RPS14; S14; EMTB
• Reactivity: Mouse
• Synonyms: 40S ribosomal protein S14; Mouse 40S ribosomal protein S14 ELISA Kit; Rps14/40S ribosomal protein S14; Rps14 elisa kit

• Reactivity: Mouse
• Sequence Length: 151

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 78-5000pg/ml
• Sensitivity: 46.9pg/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for Rps14 elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. anti-Mouse Rps14 antibody was pre-coated onto 96-well plates. And the biotin conjugated anti-Mouse Rps14 antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the Mouse Rps14 amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of Mouse Rps14 can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 68160915
• NCBI GeneID: 6208
• NCBI Accession #: NP_001020241.1
• NCBI GenBank Nucleotide #: NM_001025070.1
• Molecular Weight: 16,273 Da
• NCBI Official Full Name: 40S ribosomal protein S14
• NCBI Official Synonym Full Names: ribosomal protein S14
• NCBI Official Symbol: RPS14
• NCBI Official Synonym Symbols: S14; EMTB
• NCBI Protein Information: 40S ribosomal protein S14
• UniProt Protein Name: 40S ribosomal protein S14
• Protein Family: 40S ribosomal protein
• UniProt Gene Name: RPS14
• UniProt Entry Name: RS14_HUMAN

NCBI Description

Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RPS14: a ribosomal protein that is a component of the 40S subunit. The protein belongs to the S11P family of ribosomal proteins. It is located in the cytoplasm. Transcript variants utilizing alternative transcription initiation sites have been described in the literature. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome. In Chinese hamster ovary cells, mutations in this gene can lead to resistance to emetine, a protein synthesis inhibitor. Multiple alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Protein type: RNA-binding; Translation; Ribosomal

Chromosomal Location of Human Ortholog: 5q31-q33

Cellular Component: cytoplasm; cytosol; focal adhesion; membrane; mitochondrion; nucleolus

Molecular Function: mRNA 5'-UTR binding; protein binding; RNA binding; structural constituent of ribosome; translation regulator activity

Biological Process: cellular protein metabolic process; erythrocyte differentiation; gene expression; maturation of SSU-rRNA; maturation of SSU-rRNA from tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA); mRNA catabolic process, nonsense-mediated decay; negative regulation of transcription from RNA polymerase II promoter; regulation of translation; ribosomal small subunit assembly and maintenance; selenium metabolic process; selenocysteine metabolic process; SRP-dependent cotranslational protein targeting to membrane; translation; translational elongation; translational initiation; translational termination; viral infectious cycle; viral reproduction; viral transcription

Disease: Chromosome 5q Deletion Syndrome