Mouse anti-Human CXADR Monoclonal Antibody | anti-CXADR antibody

Mouse Monoclonal (IgG1) to Human CXADR

Cat. Num. AAA245710

• Gene Names: CXADR; CAR; HCAR; CAR4/6
• Reactivity: Human
• Applications: Immunohistochemistry, Immunocytochemistry, Western Blot
• Purity: Protein G Purified
• Synonyms: CXADR; Monoclonal Antibody; Mouse Monoclonal (IgG1) to Human CXADR; Anti-CXADR Antibody IHC-plus; CAR4/6; Coxsackie-adenovirus receptor; CVB3-binding protein; CAR; HCAR; HCVADR; Human CXADR; anti-CXADR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG1
• Specificity: Recognizes human CAR at ~46kD. Species Crossreactivity: mouse and rat.
• Purity/Purification: Protein G Purified
• Form/Format: Tris-glycine. azide, glycerol free.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 365

• Applicable Applications for anti-CXADR antibody: Immunohistochemistry (IHC - Paraffin), Immunocytochemistry (ICC), Western Blot (WB)
• Application Notes: ICC (1 - 2 ug/ml), IHC-P (5 ug/ml), WB (0.2 - 2 ug/ml); Usage: Suitable for Western Blot and Immunocytochemistry. Western Blot: 0.2-2 ug/ml. Detects CAR in lysates of CHO cells transfected with hCAR and mouse small intestine lysates. Immunocytochemistry: 1-2 ug/ml. Positive immunostaining for CAR in HeLa cells,...
• Target Species: Human
• Immunogen Description: Native human Coxsackie virus adenovirus receptor protein.
• Immunogen Type: Purified protein
• Immunogen: CXADR antibody was raised against native human Coxsackie virus adenovirus receptor protein.
• Preparation and Storage: Long term: -20 degree C; Short term: +4 degree C. Avoid repeat freeze-thaw cycles.

Immunohistochemistry (IHC)

(Anti-CXADR antibody IHC of human prostate. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 5 ug/ml.)

Related Product Information for anti-CXADR antibody

The Coxsackievirus and adenovirus receptor (CAR) is a membrane glycoprotein with a cytoplasmic domain, a transmembrane domain and an extracellular region consisting of two immunoglobulin-like domains, an amino-terminal immunoglobulin variable (IgV)-related domain (D1), which is distal to the cell surface, and a proximal IgC2 domain (D2). The Coxsackievirus and adenovirus receptor has been shown to exhibit tumor suppression activity in human bladder and prostate cancer cells.

NCBI and Uniprot Product Information

• NCBI GI #: 4503173
• NCBI GeneID: 1525
• NCBI Accession #: NP_001329.1
• NCBI GenBank Nucleotide #: NM_001338.4
• UniProt Accession #: P78310; O00694; Q8WWT6; Q8WWT7; Q8WWT8; Q9UKV4; B2R8V8; B7WPI3; D3YHP0
• Molecular Weight: 35,570 Da
• NCBI Official Full Name: coxsackievirus and adenovirus receptor isoform 1
• NCBI Official Synonym Full Names: coxsackie virus and adenovirus receptor
• NCBI Official Symbol: CXADR
• NCBI Official Synonym Symbols: CAR; HCAR; CAR4/6
• NCBI Protein Information: coxsackievirus and adenovirus receptor; HCVADR; CVB3-binding protein; coxsackievirus B-adenovirus receptor; 46 kD coxsackievirus and adenovirus receptor (CAR) protein
• UniProt Protein Name: Coxsackievirus and adenovirus receptor
• Protein Family: CXADR-like membrane protein
• UniProt Gene Name: CXADR
• UniProt Synonym Gene Names: CAR; CAR; hCAR
• UniProt Entry Name: CXAR_HUMAN

NCBI Description

The protein encoded by this gene is a type I membrane receptor for group B coxsackieviruses and subgroup C adenoviruses. Several transcript variants encoding different isoforms have been found for this gene. Pseudogenes of this gene are found on chromosomes 15, 18, and 21. [provided by RefSeq, May 2011]

Uniprot Description

CXADR: a component of the epithelial apical junction complex that is essential for the tight junction integrity. Proposed to function as a homophilic cell adhesion molecule. Recruits MPDZ to intercellular contact sites. Probably involved in transepithelial migration of polymorphonuclear leukocytes (PMN) through adhesive interactions with AMICA1 located in the plasma membrane of PMN.

Protein type: Receptor, misc.; Cell adhesion; Membrane protein, integral

Chromosomal Location of Human Ortholog: 21q21.1

Cellular Component: extracellular space; neuron projection; protein complex; tight junction; integral to plasma membrane; basolateral plasma membrane; apicolateral plasma membrane; acrosome; extracellular region; intercellular junction; lipid raft; nucleoplasm; adherens junction; growth cone; plasma membrane; cell junction; neuromuscular junction; filopodium

Molecular Function: integrin binding; viral receptor activity; identical protein binding; protein binding; beta-catenin binding; cell adhesion molecule binding; PDZ domain binding; receptor binding

Biological Process: intercellular junction assembly and maintenance; mitochondrion organization and biogenesis; neutrophil chemotaxis; entry of virus into host cell; regulation of immune response; heart development; gamma-delta T cell activation; heterophilic cell adhesion; cardiac muscle fiber development; germ cell migration; homotypic cell-cell adhesion; actin cytoskeleton reorganization; blood coagulation; leukocyte migration; defense response to virus