Western Blot (WB)
(Lysates of human heart, liver, and kidney were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml of MBS600064, as described in Protocol for Western Blot.)
Mouse anti-Human CaMKIId Monoclonal Antibody | anti-CaMKII antibody
CaMKIId (Calcium/calmodulin-dependent Protein Kinase Type II delta)
Gene Names
CaMKII; Ca2+/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase II; Cam; CaM; CaM II; CaM KII; CaM kinase; CaMK II; CaMK-II; camKII; Camkii; CamKII; CAMKII; CamKIIalpha; CAMKIIalpha; CA
Reactivity
Human
Applications
ELISA, Western Blot
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
CaMKIId; Monoclonal Antibody; CaMKIId (Calcium/calmodulin-dependent Protein Kinase Type II delta); Anti -CaMKIId (Calcium/calmodulin-dependent Protein Kinase Type II delta); anti-CaMKII antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG2B
Clone Number
8B3
Specificity
Recognizes endogenous human CaMKIId isoforms. Does not detect recombinant human CaMKIIa, CaMKIIb or CaMKIIg. Species sequence homology: mouse and rat
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 200ul sterile PBS.
Applicable Applications for anti-CaMKII antibody
ELISA (EL/EIA), Western Blot (WB)
Application Notes
Suitable for use in ELISA and Western Blot.
Dilution: Western Blot: 1ug/ml using Human heart, liver and kidney tissue.
Dilution: Western Blot: 1ug/ml using Human heart, liver and kidney tissue.
Immunogen
Recombinant protein corresponding to aa402-478 from human CaMKIId, expressed in E.coli (Q13557)
Hybridoma
Mouse myeloma with B cells obtained from a mouse immunized with purified, E. coli-derived recombinant human CaMKIId
Preparation and Storage
Lyophilized powder may be stored at -20 degree C. Stable for 12 months at -20 degree C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(Lysates of human heart, liver, and kidney were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml of MBS600064, as described in Protocol for Western Blot.)
Western Blot (WB)
(Lysates of human heart, liver, and kidney were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted with 1ug/ml of MBS600064, as described in Protocol for Western Blot.)
Related Product Information for anti-CaMKII antibody
Calcium/calmodulin-dependent protein kinase type II delta (CaMKIId) belongs to a family of multifunctional serine/threonine kinases activated in response to increases in intracellular calcium. There are 4 CaMKII isozymes, alpha, beta, gamma, and delta, and each can yield several isoforms through alternative splicing. CaMKII isoforms assemble into homo- or heteromultimeric holoenzymes composed of 8 to 12 subunits. CaMKIId expression is ubiquitous and has been identified as the predominant CaMKII isozyme in the heart, where it is implicated in the development of cardiac hypertrophy and heart failure.
Product Categories/Family for anti-CaMKII antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
Calcium/calmodulin-dependent protein kinase II, isoform M
NCBI Official Synonym Full Names
Calcium/calmodulin-dependent protein kinase II
NCBI Official Symbol
CaMKII
NCBI Official Synonym Symbols
Ca2+/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase II; Cam; CaM; CaM II; CaM KII; CaM kinase; CaMK II; CaMK-II; camKII; Camkii; CamKII; CAMKII; CamKIIalpha; CAMKIIalpha; CA
NCBI Protein Information
CG18069 gene product from transcript CG18069-RK; CG18069-PA; CG18069-PB; CG18069-PC; CG18069-PD; CG18069-PE; CG18069-PG; CG18069-PH; CG18069-PI; CG18069-PJ; CG18069-PK; CG18069-PL; CG18069-PM; CaM kinase; CaM kinase II; CaM-kinase-II; CaMKII-PA; CaMKII-PB; CaMKII-PC; CaMKII-PD; CaMKII-PE; CaMKII-PG; CaMKII-PH; CaMKII-PI; CaMKII-PJ; CaMKII-PK; CaMKII-PL; CaMKII-PM; Ca[2+]/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; Calcium/calmodulin-dependent protein kinase II; calcium/ calmodulin-dependent protein kinase II; calcium/calmodulin-dependent protein kinase II; calmodulin kinase II; calmodulin-dependent kinase; lethal (4) 102EFb
Protein Family
Research Articles on CaMKII
Similar Products
Product Notes
The CaMKII (Catalog #AAA600064) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CaMKIId (Calcium/calmodulin-dependent Protein Kinase Type II delta) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CaMKIId can be used in a range of immunoassay formats including, but not limited to, ELISA (EL/EIA), Western Blot (WB). Suitable for use in ELISA and Western Blot. Dilution: Western Blot: 1ug/ml using Human heart, liver and kidney tissue. Researchers should empirically determine the suitability of the CaMKII for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CaMKIId, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
