Typical Testing Data/Standard Curve (for reference only)
Human Ca2+/calmodulin dependent protein kinase ELISA Kit | CaM kinase elisa kit
Human Ca2+/calmodulin dependent protein kinase ELISA Kit
Gene Names
CaMKII; Ca2+/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase II; Cam; CaM; CaM II; CaM KII; CaM kinase; CaMK II; CaMK-II; camKII; Camkii; CamKII; CAMKII; CamKIIalpha; CAMKIIalpha; CA
Reactivity
Human
Synonyms
Ca2+/calmodulin dependent protein kinase; Human Ca2+/calmodulin dependent protein kinase ELISA Kit; CaM kinase elisa kit
Reactivity
Human
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
1.0 ng/mL.
Preparation and Storage
Store all reagents at 2-8 degree C.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for CaM kinase elisa kit
Intended Uses: This CALPRO ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Porcine CALPRO. This ELISA kit for research use only!
Principle of the Assay: CALPRO ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CALPRO antibody and an CALPRO-HRP conjugate. The assay sample and buffer are incubated together with CALPRO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALPRO concentration since CALPRO from samples and CALPRO-HRP conjugate compete for the anti-CALPRO antibody binding site. Since the number of sites is limited, as more sites are occupied by CALPRO from the sample, fewer sites are left to bind CALPRO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALPRO concentration in each sample is interpolated from this standard curve.
Principle of the Assay: CALPRO ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-CALPRO antibody and an CALPRO-HRP conjugate. The assay sample and buffer are incubated together with CALPRO-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CALPRO concentration since CALPRO from samples and CALPRO-HRP conjugate compete for the anti-CALPRO antibody binding site. Since the number of sites is limited, as more sites are occupied by CALPRO from the sample, fewer sites are left to bind CALPRO-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CALPRO concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
55,482 Da
NCBI Official Full Name
Calcium/calmodulin-dependent protein kinase II, isoform M
NCBI Official Synonym Full Names
Calcium/calmodulin-dependent protein kinase II
NCBI Official Symbol
CaMKII
NCBI Official Synonym Symbols
Ca2+/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; calcium/calmodulin-dependent protein kinase II; Cam; CaM; CaM II; CaM KII; CaM kinase; CaMK II; CaMK-II; camKII; Camkii; CamKII; CAMKII; CamKIIalpha; CAMKIIalpha; CA
NCBI Protein Information
CG18069 gene product from transcript CG18069-RK; CG18069-PA; CG18069-PB; CG18069-PC; CG18069-PD; CG18069-PE; CG18069-PG; CG18069-PH; CG18069-PI; CG18069-PJ; CG18069-PK; CG18069-PL; CG18069-PM; Ca2+ calmodulin-dependent kinase II; CaM kinase; CaM kinase II; CaM-kinase-II; CaMKII-PA; CaMKII-PB; CaMKII-PC; CaMKII-PD; CaMKII-PE; CaMKII-PG; CaMKII-PH; CaMKII-PI; CaMKII-PJ; CaMKII-PK; CaMKII-PL; CaMKII-PM; Ca[2+]/calmodulin-dependent protein kinase II; Calcium/calmodulin-dependent protein kinase; Calcium/calmodulin-dependent protein kinase II; calcium/ calmodulin-dependent protein kinase II; calcium/calmodulin-dependent protein kinase II; calmodulin kinase II; lethal (4) 102EFb
UniProt Protein Name
Calcium/calmodulin-dependent protein kinase type II alpha chain
UniProt Gene Name
CaMKII
UniProt Synonym Gene Names
CaM; CG18069; CaM-kinase II alpha chain
UniProt Entry Name
KCC2A_DROME
Uniprot Description
A key regulator of plasticity in synaptic physiology and behavior, alterations in its activity produce pleiotrophic effects that involve synaptic transmission and development as well as various aspects of behavior. Directly modulates eag potassium channels.
Research Articles on CaM kinase
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Product Notes
The Human CaM kinase camkii (Catalog #AAA752532) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA752532 ELISA Kit recognizes Human CaM kinase. It is sometimes possible for the material contained within the vial of "Ca2+/calmodulin dependent protein kinase, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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