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Western Blot (WB) (Western Blot: Sample: Recombinant Calcitonin, Rat.)

Mouse Calcitonin (CT) Monoclonal Antibody | anti-CT antibody

Monoclonal Antibody to Calcitonin (CT)

Gene Names
Calca; CAL6; CGRP; Cal1; Calc; RATCAL6; calcitonin
Reactivity
Rattus norvegicus (Rat)
Applications
Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Protein A + Protein G affinity chromatography
Synonyms
Calcitonin (CT); Monoclonal Antibody; Monoclonal Antibody to Calcitonin (CT); CALCA; CALC1; CGRP-I; CGRP1; KC; CCP; PDN-21; Katacalcin; Calcitonin-Related Polypeptide Alpha; Calcitonin carboxyl-terminal peptide; anti-CT antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Rattus norvegicus (Rat)
Clonality
Monoclonal
Isotype
IgG1 Kappa
Clone Number
C4
Purity/Purification
Protein A + Protein G affinity chromatography
Form/Format
Supplied as solution form in 0.01M PBS, pH7.4, containing 0.05% Proclin-300, 50% glycerol
Concentration
1mg/ml (varies by lot)
Applicable Applications for anti-CT antibody
Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP).
Application Notes
Western blotting: 0.5-2ug/mL
Immunohistochemistry: 5-20ug/mL
Immunocytochemistry: 5-20ug/mL
Optimal working dilutions must be determined by end user.
Source
Monoclonal antibody preparation
Traits
Liquid
Immunogen
Recombinant Calcitonin (Cys85~Pro116) expressed in E.coli (MBS2029875).
Preparation and Storage
Storage:
Avoid repeated freeze/thaw cycles.
Store at 4 degree C for frequent use.
Aliquot and store at -20 degree C for 24 months.

Stability Test:
thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

Western Blot (WB)

(Western Blot: Sample: Recombinant Calcitonin, Rat.)

Western Blot (WB) (Western Blot: Sample: Recombinant Calcitonin, Rat.)

Immunohistochemistry (IHC)

(DAB staining on IHC-P; Samples: Rat Testis Tissue.)

Immunohistochemistry (IHC) (DAB staining on IHC-P; Samples: Rat Testis Tissue.)

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
NCBI Official Full Name
calcitonin gene-related peptide 1 isoform Calca preproprotein
NCBI Official Synonym Full Names
calcitonin-related polypeptide alpha
NCBI Official Symbol
Calca
NCBI Official Synonym Symbols
CAL6; CGRP; Cal1; Calc; RATCAL6; calcitonin
NCBI Protein Information
calcitonin gene-related peptide 1
UniProt Protein Name
Calcitonin
UniProt Gene Name
Calca
UniProt Synonym Gene Names
Calc

NCBI Description

an inhibitor of lactotroph function and a potent vasodilator [RGD, Feb 2006]

Uniprot Description

CALCA: Calcitonin causes a rapid but short-lived drop in the level of calcium and phosphate in blood by promoting the incorporation of those ions in the bones. Belongs to the calcitonin family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1q35

Cellular Component: axon; cell soma; cytoplasm; extracellular region; extracellular space; intracellular; neuron projection; nucleus; terminal bouton

Molecular Function: calcitonin receptor binding; hormone activity; identical protein binding; neuropeptide hormone activity; protein complex binding; receptor binding

Biological Process: activation of protein kinase activity; adenylate cyclase activation; adenylate cyclase-activating G-protein coupled receptor signaling pathway; aging; cellular calcium ion homeostasis; detection of temperature stimulus involved in sensory perception of pain; elevation of cytosolic calcium ion concentration; elevation of cytosolic calcium ion concentration during G-protein signaling, coupled to IP3 second messenger (phospholipase C activating); embryo implantation; endothelial cell migration; endothelial cell proliferation; feeding behavior; G-protein coupled receptor internalization; inflammatory response; leukocyte adhesion; monocyte chemotaxis; negative regulation of blood pressure; negative regulation of bone resorption; negative regulation of neurological process; negative regulation of ossification; negative regulation of osteoclast differentiation; negative regulation of smooth muscle contraction; negative regulation of transcription, DNA-dependent; neurological system process involved in regulation of systemic arterial blood pressure; neuromuscular junction development; neuropeptide signaling pathway; positive regulation of adenylate cyclase activity; positive regulation of cAMP biosynthetic process; positive regulation of cAMP metabolic process; positive regulation of cell adhesion; positive regulation of interleukin-1 alpha production; positive regulation of interleukin-8 production; positive regulation of macrophage differentiation; positive regulation of ossification; protein amino acid phosphorylation; receptor internalization; regulation of blood pressure; regulation of cytosolic calcium ion concentration; regulation of heart contraction; regulation of heart rate; response to heat; response to pain; vasculature development; vasodilation; vasodilation of artery during baroreceptor response to increased systemic arterial blood pressure

Research Articles on CT

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Product Notes

The CT calca (Catalog #AAA2090546) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to Calcitonin (CT) reacts with Rattus norvegicus (Rat) and may cross-react with other species as described in the data sheet. AAA Biotech's Calcitonin (CT) can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB); Immunohistochemistry (IHC); Immunocytochemistry (ICC); Immunoprecipitation (IP). Western blotting: 0.5-2ug/mL Immunohistochemistry: 5-20ug/mL Immunocytochemistry: 5-20ug/mL Optimal working dilutions must be determined by end user. Researchers should empirically determine the suitability of the CT calca for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Calcitonin (CT), Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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