Mouse anti-Human C5aR Monoclonal Antibody | anti-C5aR antibody

Anti-C5aR [32-G1]

Cat. Num. AAA488756

• Gene Names: C5AR1; C5A; C5AR; C5R1; CD88
• Reactivity: Human
• Applications: Western Blot, Immunofluorescence
• Purity: Purified antibody. Protein A affinity purified
• Synonyms: C5aR; Monoclonal Antibody; Anti-C5aR [32-G1]; CD88; C5a anaphylatoxin chemotactic receptor; C5a anaphylatoxin chemotactic receptor 1; C5a-R; pC5aR; 32G-1; anti-C5aR antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Mouse IgG1, kappa
• Clone Number: 32-G1
• Specificity: The 32-G1 antibody recognizes C5aR which has its serine 334 phosphorylated. The mAb 32-G1 reacted specifically with a C5aR peptide that contained phosphoserine 334 and also reacted with C5aRs that were isolated from C5a-stimulated, transfected RBL-C5aR cells. It did not Recognise a receptor mutant (C5aR-S-) with alanine replacement of all six C-terminal serine phosphorylation sites (Pollok-Kopp et al., 2007). Antibody 32-G1 specifically reacts with C5aR phosphoserine 334, independent of the phosphorylation state of the neighboring serine residues (Pollok-Kopp et al., 2007). Studies with Bt2cAMP-treated U937 cells show that 32-G1 specifically reacts with ligand-activated C5aR at endogenous receptor expression levels but only weakly recognizes C5aR isolated from unstimulated cells (Pollok-Kopp et al., 2007).
• Purity/Purification: Purified antibody. Protein A affinity purified
• Form/Format: PBS with 0.02% Proclin 300.
• Concentration: 1mg/ml (varies by lot)

• Applicable Applications for anti-C5aR antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: The 32-G1 antibody is recommended for the detection and analysis of C5aR (CD88) phosphorylated on Ser-334. For instance, this antibody was successfully used in the identification of C5aR protein in a U937 cell lysate via Western blot (Pollok-Kopp et al., 2007).
• PMID: 17145764
• Preparation and Storage: Store at 4 degree C for up to 3 months. For longer storage, aliquot and store at -20 degree C.

Immunofluorescence (IF)

(Immunofluorescence staining of fixed U937 cells with anti-C5aR antibody 32-G1 (MBS488757) Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix coverslips stained with the chimeric rabbit IgG version 32-G1 (MBS488757) at 10ug/ml for 1h followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom MBS488757, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (MBS488140) followed by staining with Alexa Fluor 488 secondary antibody.)

Flow Cytometry (FC/FACS)

(Flow cytometry using the Anti-C5aR antibody 32-G1 (MBS488757). U937 cells were stained with anti-unknown specificity antibody (MBS488149; isotype control, black line) or the rabbit IgG version of 32-G1 (MBS488757, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.)

Related Product Information for anti-C5aR antibody

Recombinant monoclonal antibody to C5aR. Recombinant Platform with variable regions (i.e. specificity) from the hybridoma 32-G1.
This antibody was raised by immunizing mice a synthetic C5aR C-terminal phosphopeptide that contained phosphoserine instead of serine at position 334 and also at the neighboring phosphorylation sites Ser-332 and Ser-338.

Product Categories/Family for anti-C5aR antibody

complement; C5a; immunology; innate immunity; phosphorylation

References

Pollok-Kopp et al. Dynamics of protein kinase C-mediated phosphorylation of the complement C5a receptor on serine 334. J Biol Chem. 2007 Feb 16;282(7):4345-53. Epub 2006 Dec 4.
Note on publication: This article describes the generation and characterization of the 32-G1 antibody.

NCBI and Uniprot Product Information

• NCBI GI #: 4502509
• NCBI GeneID: 728
• NCBI Accession #: NP_001727.1
• NCBI GenBank Nucleotide #: NM_001736.3
• UniProt Accession #: P21730
• Molecular Weight: 39,336 Da
• NCBI Official Full Name: C5a anaphylatoxin chemotactic receptor
• NCBI Official Synonym Full Names: complement component 5a receptor 1
• NCBI Official Symbol: C5AR1
• NCBI Official Synonym Symbols: C5A; C5AR; C5R1; CD88
• NCBI Protein Information: C5a anaphylatoxin chemotactic receptor; C5a-R; C5a ligand; C5a anaphylatoxin receptor; complement component 5 receptor 1
• UniProt Protein Name: C5a anaphylatoxin chemotactic receptor
• UniProt Gene Name: C5AR1
• UniProt Synonym Gene Names: C5AR; C5R1; C5a-R; C5aR
• UniProt Entry Name: C5AR_HUMAN

Uniprot Description

C5aR: a family 1 G-protein coupled receptor that stimulates the GTPase activity of Gi2. Receptor for the chemotactic and inflammatory complement factor C5a. Stimulates chemotaxis, granule enzyme release and superoxide anion production. Phosphorylated in response to C5a or after stimulation of cells with phorbol esters. Expressed on monocytes, granulocytes, dendritic cells, astrocytes and microglia.

Protein type: GPCR, family 1; Membrane protein, multi-pass; Membrane protein, integral; Receptor, GPCR

Chromosomal Location of Human Ortholog: 19q13.3-q13.4

Cellular Component: cell surface; basolateral plasma membrane; integral to plasma membrane; apical part of cell; cytoplasmic membrane-bound vesicle; plasma membrane

Molecular Function: complement component C5a receptor activity; complement component C5a binding; C5a anaphylatoxin receptor activity

Biological Process: neutrophil chemotaxis; organ regeneration; sensory perception of chemical stimulus; activation of MAPK activity; apoptosis; response to lipopolysaccharide; chemotaxis; cell proliferation in hindbrain; signal transduction; mRNA transcription from RNA polymerase II promoter; elevation of cytosolic calcium ion concentration; defense response to Gram-positive bacterium; phospholipase C activation; response to peptidoglycan; cellular defense response; immune response; negative regulation of neuron apoptosis; inflammatory response; positive regulation of epithelial cell proliferation