Mouse C-Terminal Binding Protein 1 Monoclonal Antibody | anti-CTBP1 antibody

Mouse Anti Human C-Terminal Binding Protein 1

Cat. Num. AAA140346

• Gene Names: CTBP1; BARS
• Applications: ELISA, Western Blot
• Purity: Recombinant human CTBP1 (1-440aa) purified from E. coli
• Synonyms: C-Terminal Binding Protein 1; Monoclonal Antibody; Mouse Anti Human C-Terminal Binding Protein 1; CTBP1 Antibody; Monoclonal Mouse Anti Human Antibody; BARS; Brefeldin A-Ribosylated Substrate; MGC104684; CtBP1; anti-CTBP1 antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG2b heavy chain and Kappa light chain
• Clone Number: AT4D6
• Purity/Purification: Recombinant human CTBP1 (1-440aa) purified from E. coli
• Form/Format: 1mg/ml containing Phosphate-Buffered Saline (pH 7.4) with 0.1% Sodium Azide.
• Sequence Length: 429

• Applicable Applications for anti-CTBP1 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: The antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500.Recommended starting dilution is 1:500
• Immunogen: Anti-human CTBP1 mAb, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CTBP1 protein.
• Preparation and Storage: For periods up to 1 month store at 4 degree C, for longer periods of time, store at -20 degree C. Prevent freeze thaw cycles.; 12 months at -20 degree C. 1 month at 4 degree C.

Related Product Information for anti-CTBP1 antibody

Introduction: CtBP was initially known as a cellular protein which cooperates with a C-terminal motif of adenovirus E1A oncoproteins. C-terminal-binding protein is extremely conserved in higher eukaryotes. Vertebrates hold two highly homologous CtBP proteins (CtBP1 and CtBP2) while invertebrates h a single CtBP. CtBP family proteins has a significant sequence homology with D-2-hydroxy acid dehydrogenases. In addition, CtBP proteins cooperates with numerous proteins and functions as a co-repressor of transcription.

Product Categories/Family for anti-CTBP1 antibody

Monoclonal/Polyclonal Antibodies; Monoclonal Antibodies

NCBI and Uniprot Product Information

• NCBI GI #: 61743967
• NCBI GeneID: 1487
• NCBI Accession #: NP_001012632.1
• NCBI GenBank Nucleotide #: NM_001012614.1
• Molecular Weight: 46,405 Da
• NCBI Official Full Name: C-terminal-binding protein 1 isoform 2
• NCBI Official Synonym Full Names: C-terminal binding protein 1
• NCBI Official Symbol: CTBP1
• NCBI Official Synonym Symbols: BARS
• NCBI Protein Information: C-terminal-binding protein 1; brefeldin A-ribosylated substrate
• UniProt Protein Name: C-terminal-binding protein 1
• UniProt Gene Name: CTBP1
• UniProt Synonym Gene Names: CTBP; CtBP1
• UniProt Entry Name: CTBP1_HUMAN

NCBI Description

This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]

Uniprot Description

CTBP1: Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation. Corepressor targeting diverse transcription regulators such as GLIS2. Has dehydrogenase activity. Homo- or heterodimer. Heterodimer with CTBP2. Interacts with PRDM16; the interaction represses white adipose tissue (WAT)- specific genes expression. Interacts with GLIS2, FOXP2, HDAC4, HDAC5, HDAC9 and ZNF217. Interacts with adenovirus E1A protein (via its C-terminus); the interaction disrupts the interaction of CTBP1 with RBBP8. Interacts with Epstein-Barr virus EBNA3 and EBNA6. Interacts with ELK3 (via its PXDLS motif). Interacts with RBBP8 (via its PXDLS motif); the interaction is disrupted by binding to adenovirus E1A. Interacts with FOXP1, HIPK2, PNN, NRIP1, MECOM, ZNF366, ZFHX1B and WIZ. Interaction with SATB1 (non-acetylated form); the interaction stabilizes its attachment to DNA and promotes transcription repression. Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Nuclear receptor co-regulator; Transcription, coactivator/corepressor; EC 1.1.1.-; Oxidoreductase

Chromosomal Location of Human Ortholog: 4p16

Cellular Component: nucleoplasm; transcription factor complex; transcriptional repressor complex; cytosol; nucleus

Molecular Function: protein C-terminus binding; protein domain specific binding; protein binding; protein homodimerization activity; NAD binding; transcription factor binding; transcription factor activity; oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor

Biological Process: negative regulation of cell proliferation; positive regulation of histone deacetylation; transcription, DNA-dependent; regulation of cell cycle; white fat cell differentiation; negative regulation of transcription from RNA polymerase II promoter; viral genome replication; negative regulation of transcription, DNA-dependent; protein amino acid phosphorylation; Golgi organization and biogenesis; negative regulation of histone acetylation

Disease: Wolf-hirschhorn Syndrome