Rabbit anti-Human RGS19 Polyclonal Antibody | anti-RGS19 antibody

RGS19 Antibody (S24)

Cat. Num. AAA9217097

• Gene Names: RGS19; GAIP; RGSGAIP
• Reactivity: Human
• Applications: Western Blot, Immunohistochemistry, ELISA
• Synonyms: RGS19; Polyclonal Antibody; RGS19 Antibody (S24); Regulator of G-protein signaling 19; G-alpha-interacting protein; GAIP; GNAI3IP; anti-RGS19 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Ig
• Specificity: This RGS19 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 9~39 amino acids surrounding S24 of human RGS19.
• Form/Format: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
• Sequence Length: 217

• Applicable Applications for anti-RGS19 antibody: Western Blot (WB), Immunohistochemistry (IHC) Paraffin, ELISA (EIA)
• Application Notes: WB:~~1:1000; IHC-P:~~1:10~50
• Subtitle: Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
• Cellular Location: Membrane; Lipid-anchor.
• Tissue Location: Highest expression in lung. Placenta, liver and heart also express high levels of GAIP.
• Antigen Region: 9-39 aa
• Preparation and Storage: Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(All lanes : Anti-RGS19 Antibody (S24) at 1:1000 dilutionLane 1: human lung lysateLane 2: human placenta lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 25 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Immunohistochemistry (IHC)-Paraffin

(Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RGS19 Antibody (S24), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Related Product Information for anti-RGS19 antibody

Description: RGS19 enhances the intrinsic GTPase-activating protein activity of the Galphai3 protein, which stimulates autophagy by favoring the GDP-bound form of Galphai3. Macroautophagy is the major inducible pathway for the general turnover of cytoplasmic constituents in eukaryotic cells, it is also responsible for the degradation of active cytoplasmic enzymes and organelles during nutrient starvation. Macroautophagy involves the formation of double-membrane bound autophagosomes which enclose the cytoplasmic constituent targeted for degradation in a membrane bound structure, which then fuse with the lysosome (or vacuole) releasing a single-membrane bound autophagic bodies which are then degraded within the lysosome (or vacuole).

Background: Inhibits signal transduction by increasing the GTPase activity of G protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the order G(i)a3 > G(i)a1 > G(o)a >> G(z)a/G(i)a2. Activity on G(z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein.

Product Categories/Family for anti-RGS19 antibody

Primary Antibodies; Cancer; Cardiovascular; Metabolism; Signal Transduction

References

Baehrecke EH. Nat Rev Mol Cell Biol. 6 (6):505-10. (2005). Lum JJ, et al. Nat Rev Mol Cell Biol. 6 (6):439-48. (2005). Greenberg JT. Dev Cell. 8 (6):799-801. (2005). Levine B. Cell. 120 (2):159-62. (2005). Shintani T and Klionsky DJ. Science. 306 (5698):990-5. (2004). de Vries L., et al. PNAS 93:15203-15208 (1996). de Alba E., et al. J. Mol. Biol. 291:927-939 (1999). Wang J., et al. J. Biol. Chem. 273:26014-26025 (1998). Ogier-Denis E., et al. J. Biol. Chem. 275:39090-39095 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 86990435
• NCBI GeneID: 10287
• NCBI Accession #: NP_001034556.1
• NCBI GenBank Nucleotide #: NM_001039467.1
• UniProt Accession #: P49795; Q53XN0; Q8TD60; A8K216; E1P5G9
• Molecular Weight: 24636 Da
• NCBI Official Full Name: regulator of G-protein signaling 19
• NCBI Official Synonym Full Names: regulator of G-protein signaling 19
• NCBI Official Symbol: RGS19
• NCBI Official Synonym Symbols: GAIP; RGSGAIP
• NCBI Protein Information: regulator of G-protein signaling 19
• UniProt Protein Name: Regulator of G-protein signaling 19
• Protein Family: Regulator of G-protein signaling
• UniProt Gene Name: RGS19
• UniProt Synonym Gene Names: GAIP; GNAI3IP; RGS19; GAIP
• UniProt Entry Name: RGS19_HUMAN

NCBI Description

G proteins mediate a number of cellular processes. The protein encoded by this gene belongs to the RGS (regulators of G-protein signaling) family and specifically interacts with G protein, GAI3. This protein is a guanosine triphosphatase-activating protein that functions to down-regulate Galpha i/Galpha q-linked signaling. Alternatively spliced transcript variants encoding the same protein isoform have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

RGS19: inhibits signal transduction by increasing the GTPase activity of g protein alpha subunits thereby driving them into their inactive GDP-bound form. Binds to G-alpha subfamily 1 members, with the order G(I)a3 > G(I)a1 > G(O)a >> G(Z)a/G(I)a2. Activity on G(Z)-alpha is inhibited by phosphorylation and palmitoylation of the G-protein.

Protein type: GAPs, RGS; GAPs

Chromosomal Location of Human Ortholog: 20q13.33

Cellular Component: beta-catenin destruction complex; brush border; cell cortex; clathrin-coated vesicle; cytoplasmic membrane-bound vesicle; cytoplasmic microtubule; Golgi apparatus; heterotrimeric G-protein complex; lipid raft; membrane; postsynaptic density

Molecular Function: beta-catenin binding; G-protein alpha-subunit binding; GTPase activator activity; protein binding; protein kinase binding

Biological Process: autophagy; cell death; cell differentiation; dorsal/ventral axis specification; G-protein coupled receptor protein signaling pathway; positive regulation of GTPase activity; regulation of transcription, DNA-dependent; small GTPase mediated signal transduction