Western Blot (WB)
(The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: 293T cell lysate Lane 4.: AGS cell lysate Lane 5.: Raji cell lysate Lane 6.: A549 cell lysate Lane 7.: Jurkat cell lysate)
Mouse anti-Human CTBP1 Monoclonal Antibody | anti-CTBP1 antibody
CTBP1 antibody
Recommended starting dilution is 1:500
For long term storage, aliquot and store at -20 degree C.
Avoid repeated freezing and thawing cycles.
Western Blot (WB)
(The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: 293T cell lysate Lane 4.: AGS cell lysate Lane 5.: Raji cell lysate Lane 6.: A549 cell lysate Lane 7.: Jurkat cell lysate)
Western Blot (WB)
(The cell and tissue lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CTBP1 antibody (1:500). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Lane 1.: HeLa cell lysate Lane 2.: NIH3T3 cell lysate Lane 3.: 293T cell lysate Lane 4.: AGS cell lysate Lane 5.: Raji cell lysate Lane 6.: A549 cell lysate Lane 7.: Jurkat cell lysate)
Immunofluorescence (IF)
(ICC/IF analysis of CTBP1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) ICC/IF analysis of CTBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
Immunofluorescence (IF)
(ICC/IF analysis of CTBP1 in A549 cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green) ICC/IF analysis of CTBP1 in HeLa cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CTBP1 antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a protein that binds to the C-terminus of adenovirus E1A proteins. This phosphoprotein is a transcriptional repressor and may play a role during cellular proliferation. This protein and the product of a second closely related gene, CTBP2, can dimerize. Both proteins can also interact with a polycomb group protein complex which participates in regulation of gene expression during development. Alternative splicing of transcripts from this gene results in multiple transcript variants. [provided by RefSeq, Jul 2008]
Uniprot Description
Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.
Research Articles on CTBP1
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Product Notes
The CTBP1 ctbp1 (Catalog #AAA200205) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CTBP1 antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CTBP1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF). WB: 1:500. Recommended starting dilution is 1:500. Researchers should empirically determine the suitability of the CTBP1 ctbp1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CTBP1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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