Mouse anti-Human c-erbB2 Monoclonal Antibody
c-erbB2 (HER-2, neu Receptor) (BSA & Azide Free)
Reactivity
Human
Applications
Immunoprecipitation
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
c-erbB2; Monoclonal Antibody; c-erbB2 (HER-2; neu Receptor) (BSA & Azide Free); Anti -c-erbB2 (HER-2; anti-c-erbB2 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3F36
Specificity
Recognizes human c-erbB2. Inhibits the binding of EGF and NDF to their direct receptors. Inhibits tumorigenic growth. Demonstrates modest effect on c-erbB-2 internalization. Induces the tyrosine kinase activity of the c-erbB-2. Accelerates dissociation of EGF/NDF from their cellular binding sites. Inhibits mitogenic effect of EGF/NDF. Inhibits dimerization of c-erbB Receptors.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.4. No stabilizing proteins added. No preservative added. Glycerol free. Also available with BSA. See C0026-11Q.
Applicable Applications for anti-c-erbB2 antibody
Immunoprecipitation (IP)
Application Notes
Suitable for use in Immunoprecipitation. Not suitable for use in Western Blot and Immunohistochemistry.
Dilution: Inhibits Binding of EGF (~70%), NDF (~40%): 25-50ug/ml [N87 human gastric carcinoma cell line (expresses c-erbB-1, -2, and -3) and T47D human breast cancer cell line (expresses all four c-erbB receptors)]. Intact IgG as well as monovalent fragments (Fab) inhibit the binding of EGF/NDF. Inhibits the binding of EGF/NDF by interfering with the formation of c-erbB-2-containing heterodimers. The inhibitory effect is due to acceleration of ligand dissociation which results in reduction of the ability of c-erbB-2 to transactivate the mitogenic signals of EGF/NDF.
Inhibits Mitogenic Effect of EGF/NDF: 50ug/ml
Inhibits Tumor Growth (~85%): 350ug/injection given I.P. on day 3, 7, 10 post subcutaneous inoculation of N87 cells (3x10e6) in CD1/nude mice.
Internalization of c-erbB-2: 40ug/ml
Stimulation of c-erbB-2 Phosphorylation: 20ug/ml
Immunoprecipitation (Native only) (Use Protein G): 2ug/mg protein lysate
Dilution: Inhibits Binding of EGF (~70%), NDF (~40%): 25-50ug/ml [N87 human gastric carcinoma cell line (expresses c-erbB-1, -2, and -3) and T47D human breast cancer cell line (expresses all four c-erbB receptors)]. Intact IgG as well as monovalent fragments (Fab) inhibit the binding of EGF/NDF. Inhibits the binding of EGF/NDF by interfering with the formation of c-erbB-2-containing heterodimers. The inhibitory effect is due to acceleration of ligand dissociation which results in reduction of the ability of c-erbB-2 to transactivate the mitogenic signals of EGF/NDF.
Inhibits Mitogenic Effect of EGF/NDF: 50ug/ml
Inhibits Tumor Growth (~85%): 350ug/injection given I.P. on day 3, 7, 10 post subcutaneous inoculation of N87 cells (3x10e6) in CD1/nude mice.
Internalization of c-erbB-2: 40ug/ml
Stimulation of c-erbB-2 Phosphorylation: 20ug/ml
Immunoprecipitation (Native only) (Use Protein G): 2ug/mg protein lysate
Immunogen
Extracellular domain of recombinant human c-erbB-2/HER-2 oncoprotein. Cellular Localization: Cell membrane MW of antigen: 185kD Epitope: Involved in the formation of heterodimers.
Positive Control
SKBR-3 cells
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Related Product Information for anti-c-erbB2 antibody
c-erbB-2 is a receptor tyrosine kinase of the c-erbB family. Approximately 25% of primary breast and ovarian tumors were found to overexpress the protein.
Product Categories/Family for anti-c-erbB2 antibody
NCBI and Uniprot Product Information
NCBI GI #
Molecular Weight
185kD Epitope: Involved in the formation of heterodimers.
NCBI Official Full Name
Chain P, Single Chain Fv Of C219 Heavy Chain V101l Mutant In Complex With Synthetic Epitope Peptide
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Product Notes
The c-erbB2 (Catalog #AAA600214) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The c-erbB2 (HER-2, neu Receptor) (BSA & Azide Free) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's c-erbB2 can be used in a range of immunoassay formats including, but not limited to, Immunoprecipitation (IP). Suitable for use in Immunoprecipitation. Not suitable for use in Western Blot and Immunohistochemistry. Dilution: Inhibits Binding of EGF (~70%), NDF (~40%): 25-50ug/ml [N87 human gastric carcinoma cell line (expresses c-erbB-1, -2, and -3) and T47D human breast cancer cell line (expresses all four c-erbB receptors)]. Intact IgG as well as monovalent fragments (Fab) inhibit the binding of EGF/NDF. Inhibits the binding of EGF/NDF by interfering with the formation of c-erbB-2-containing heterodimers. The inhibitory effect is due to acceleration of ligand dissociation which results in reduction of the ability of c-erbB-2 to transactivate the mitogenic signals of EGF/NDF. Inhibits Mitogenic Effect of EGF/NDF: 50ug/ml Inhibits Tumor Growth (~85%): 350ug/injection given I.P. on day 3, 7, 10 post subcutaneous inoculation of N87 cells (3x10e6) in CD1/nude mice. Internalization of c-erbB-2: 40ug/ml Stimulation of c-erbB-2 Phosphorylation: 20ug/ml Immunoprecipitation (Native only) (Use Protein G): 2ug/mg protein lysate. Researchers should empirically determine the suitability of the c-erbB2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-erbB2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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