Testing Data
Mouse c-erbB2 Monoclonal Antibody
c-erbB2 (HER-2, neu Receptor) (BSA & Azide Free)
Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunoprecipitation, Immunohistochemistry
Purity
Affinity PurifiedPurified by Protein G affinity chromatography.
Synonyms
c-erbB2; Monoclonal Antibody; c-erbB2 (HER-2; neu Receptor) (BSA & Azide Free); Anti -c-erbB2 (HER-2; anti-c-erbB2 antibody
Host
Mouse
Reactivity
Human, Mouse, Rat
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3F33
Specificity
Recognizes human c-erbB-2 (HER-2, neu). Species Crossreactivity: mouse and rat.
Purity/Purification
Affinity Purified
Purified by Protein G affinity chromatography.
Purified by Protein G affinity chromatography.
Form/Format
Supplied as a liquid in 10mM PBS, pH 7.4. Also available with BSA and azide. See C0026-11N
Applicable Applications for anti-c-erbB2 antibody
Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry.
Dilution: Immunoprecipitation (Native and Denatured) (Use Protein G): 2ug/mg protein lysate
Western Blot: 1-2ug/ml for 2hr at RT
Immunohistochemistry: 1:200-1:400 for 20 minutes at RT.
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Dilution: Immunoprecipitation (Native and Denatured) (Use Protein G): 2ug/mg protein lysate
Western Blot: 1-2ug/ml for 2hr at RT
Immunohistochemistry: 1:200-1:400 for 20 minutes at RT.
Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Immunogen
Cytoplasmic domain of recombinant human c-erbB-2/HER-2 oncoprotein, MW: 185kD. Cellular Localization: Cell membrane.
Positive Control
SKBR-3 or T47D cells, or breast carcinoma.
Preparation and Storage
May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Testing Data
Testing Data
Testing Data
Testing Data
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast carcinoma stained with MBS601210 using peroxidase-conjugate and AEC chromogen. Note intense cell membrane staining of tumor cells.)
Immunohistochemistry (IHC)
(Formalin-fixed, paraffin-embedded human breast carcinoma stained with MBS601210 using peroxidase-conjugate and AEC chromogen. Note intense cell membrane staining of tumor cells.)
Related Product Information for anti-c-erbB2 antibody
c-erbB-2 is a receptor tyrosine kinase. It exhibits extracellular domains with two cysteine-rich sequences, and a cytoplasmic tyrosine kinase domain flanked by large hydrophilic tails that carry several tyrosine autophosphorylation sites. Approximately 25% of primary breast and ovarian tumors were found to overexpress the protein. The cerbB-2 protein is overexpressed in a variety of carcinomas especially those of breast and ovary.
Product Categories/Family for anti-c-erbB2 antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
Chain P, Single Chain Fv Of C219 Heavy Chain V101l Mutant In Complex With Synthetic Epitope Peptide
Similar Products
Product Notes
The c-erbB2 (Catalog #AAA601210) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The c-erbB2 (HER-2, neu Receptor) (BSA & Azide Free) reacts with Human, Mouse, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's c-erbB2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC). Suitable for use in Immunoprecipitation, Western Blot and Immunohistochemistry. Dilution: Immunoprecipitation (Native and Denatured) (Use Protein G): 2ug/mg protein lysate Western Blot: 1-2ug/ml for 2hr at RT Immunohistochemistry: 1:200-1:400 for 20 minutes at RT. Note: Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes. Researchers should empirically determine the suitability of the c-erbB2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "c-erbB2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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