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Testing Data #1 (Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II (monomorphic) (MBS224486))

Mouse MHC CLASS II MONOMORPHIC Monoclonal Antibody

MOUSE ANTI BOVINE MHC CLASS II MONOMORPHIC:RPE

Applications
Flow Cytometry, Functional Assay
Synonyms
MHC CLASS II MONOMORPHIC; Monoclonal Antibody; MOUSE ANTI BOVINE MHC CLASS II MONOMORPHIC:RPE; anti-MHC CLASS II MONOMORPHIC antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
IL-A21
Form/Format
Purified IgG conjugated to R. Phycoerythrin (RPE) - lyophilised
Applicable Applications for anti-MHC CLASS II MONOMORPHIC antibody
Flow cytometry (FC/FACS)
Application Notes
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Minimum Dilution: Neat
Reconstitution
Reconstitute with 1.0 ml distilled water
Perservative Stabilisers
Fusion Partners
Spleen cells from immunised BALB/c mice were fused with cells of the X63.Ag8.653 myeloma cell line.
Buffer Solution
Target Species
Bovine
Preparation and Storage
Prior to reconstitution store at 4 degree C. After reconstitution store at 4 degree C. DO NOT FREEZE. This product should be stored undiluted. This product is photosensitive and should be protected from light. Should this product contain a precipitate we recommend microcentrifugation before use.
Shelf Life: 12 months from date of reconstitution.

Testing Data #1

(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II (monomorphic) (MBS224486))

Testing Data #1 (Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II (monomorphic) (MBS224486))

Testing Data #2

(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II (monomorphic):FITC (MBS224721))

Testing Data #2 (Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II (monomorphic):FITC (MBS224721))

Testing Data #3

(Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II (monomorphic):RPE (MBS224644))

Testing Data #3 (Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine MHC Class II (monomorphic):RPE (MBS224644))
Related Product Information for anti-MHC CLASS II MONOMORPHIC antibody
Mouse anti Bovine MHC Class II Monomorphic antibody, clone IL-A21 recognizes a monomorphic epitope within the bovine MHC II cell surface antigen. Clone IL-A21 is reported to react with an epitope which is common to both BoLA DR and DQ  (Howard et al.1997). Expression of MHC II molecules have been demonstrated on bovine B cells, activated T cells, alveolar macrophages, monocytes and mammary and bronchial epithelial cells. Clone IL-A21 is reported to inhibit T cell proliferation following FMDV infection.

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Product Notes

The MHC CLASS II MONOMORPHIC (Catalog #AAA224644) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's MHC CLASS II MONOMORPHIC can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS). Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. Flow Cytometry: Minimum Dilution: Neat. Researchers should empirically determine the suitability of the MHC CLASS II MONOMORPHIC for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "MHC CLASS II MONOMORPHIC, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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