Rabbit DDB2 Antibody | anti-DDB2 antibody

DDB2 Antibody

Cat. Num. AAA8505539

• Gene Names: DDB2; XPE; DDBB; UV-DDB2
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Purity: Affinity purification
• Synonyms: DDB2; DDB2 Antibody; DDBB; FLJ34321; UV-DDB2; anti-DDB2 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Purity/Purification: Affinity purification
• Sequence Length: 427

• Applicable Applications for anti-DDB2 antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: WB: 1:500-1:2000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human DDB2
• Storage Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
• Preparation and Storage: Store at-20 degree C or-80 degree C. Avoid freeze/thaw cycles.

Testing Data

(Western blot analysis of extracts of MCF-7cell line, using DDB2 antibody.)

Related Product Information for anti-DDB2 antibody

Damaged DNA-Binding Protein (DDB) consists of a 127 kDa subunit (DDB-1) and a 48 kDa subunit (DDB-2) that contribute to the formation of the UV-damaged DNA-binding protein complex (UV-DDB) (1-3). In conjunction with CUL4A and ROC-1, the UV-DDB complex forms an E3 ubiquitin ligase that recognizes a broad spectrum of DNA lesions such as cyclobutane pyrimidine dimers, 6-4 photoproducts, apurinic sites and short mismatches. The complex polyubiquitinates components of the nucleotide excision repair pathway (4-6). Loss of DDB activity has been identified in a subset of xerodermapigmentosum complementation group E (XP-E) patients and has been linked to the deficient repair of cyclobutane pyrimidine dimers in cells derived from these patients (7-10).

Product Categories/Family for anti-DDB2 antibody

Autophagy antibody; Cancer; Cardiovascular; Cell Biology; Epigenetics & Nuclear Signaling; Developmental Biologys; Immunology; Drug Discovery Products; Metabolism; Neuroscience; Signal Transduction; Stem Cells

References

1. Reardon, J.T. et al. (1993) J BiolChem 268, 21301-8. 2. Keeney, S. et al. (1993) J BiolChem 268, 21293-300. 3. Hwang, B.J. and Chu, G. (1993) Biochemistry 32, 1657-66. 4. Chu, G. and Chang, E. (1990) ProcNatlAcadSci USA 87, 3324-7. 5. Hirschfeld, S. et al. (1990) Mol Cell Biol 10, 2041-8. 6. Payne, A. and Chu, G. (1994) Mutat Res 310, 89-102. 7. Chu, G. and Chang, E. (1988) Science 242, 564-7. 8. Nichols, A.F. et al. (1996) J BiolChem 271, 24317-20. 9. Kataoka, H. and Fujiwara, Y. (1991) BiochemBiophys Res Commun 175, 1139-43. 10. Keeney, S. et al. (1992) Mutat Res 273, 49-56.

NCBI and Uniprot Product Information

• NCBI GI #: 4557515
• NCBI GeneID: 1643
• NCBI Accession #: NP_000098.1
• NCBI GenBank Nucleotide #: NM_000107.2
• UniProt Accession #: Q92466; Q76E54; Q76E55; Q76E56; Q76E57; B2R875
• Molecular Weight: 48kDa
• NCBI Official Full Name: DNA damage-binding protein 2 isoform WT
• NCBI Official Synonym Full Names: damage specific DNA binding protein 2
• NCBI Official Symbol: DDB2
• NCBI Official Synonym Symbols: XPE; DDBB; UV-DDB2
• NCBI Protein Information: DNA damage-binding protein 2
• UniProt Protein Name: DNA damage-binding protein 2
• Protein Family: DNA damage-binding protein
• UniProt Gene Name: DDB2
• UniProt Synonym Gene Names: DDBb; UV-DDB 2

NCBI Description

This gene encodes a protein that is necessary for the repair of ultraviolet light-damaged DNA. This protein is the smaller subunit of a heterodimeric protein complex that participates in nucleotide excision repair, and this complex mediates the ubiquitylation of histones H3 and H4, which facilitates the cellular response to DNA damage. This subunit appears to be required for DNA binding. Mutations in this gene cause xeroderma pigmentosum complementation group E, a recessive disease that is characterized by an increased sensitivity to UV light and a high predisposition for skin cancer development, in some cases accompanied by neurological abnormalities. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2014]

Uniprot Description

Required for DNA repair. Binds to DDB1 to form the UV-damaged DNA-binding protein complex (the UV-DDB complex). The UV-DDB complex may recognize UV-induced DNA damage and recruit proteins of the nucleotide excision repair pathway (the NER pathway) to initiate DNA repair. The UV-DDB complex preferentially binds to cyclobutane pyrimidine dimers (CPD), 6-4 photoproducts (6-4 PP), apurinic sites and short mismatches. Also appears to function as the substrate recognition module for the DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex DDB1-CUL4-ROC1 (also known as CUL4-DDB-ROC1 and CUL4-DDB-RBX1). The DDB1-CUL4-ROC1 complex may ubiquitinate histone H2A, histone H3 and histone H4 at sites of UV-induced DNA damage. The ubiquitination of histones may facilitate their removal from the nucleosome and promote subsequent DNA repair. The DDB1-CUL4-ROC1 complex also ubiquitinates XPC, which may enhance DNA-binding by XPC and promote NER. Isoform D1 and isoform D2 inhibit UV-damaged DNA repair.