BoTox-E Clone 209F2 Monoclonal Antibody
Anti-Botulism Toxin-E Monoclonal Antibody 209F2
Applications
Immunofluorescent Staining of Neuro2A Cells
Synonyms
BoTox-E Clone 209F2; Monoclonal Antibody; Anti-Botulism Toxin-E Monoclonal Antibody 209F2; BoTox-E (Clone 209F2); anti-BoTox-E Clone 209F2 antibody
Clonality
Monoclonal
Isotype
Mouse IgG
Clone Number
209F2
Specificity
Polypeptide ::: % Cross Reactivity
BoTox-E (2-17) ::: 100
BoTox-E
BoTox-E (2-17) ::: 100
BoTox-E
Form/Format
Supplied as Sterile Culture Supernatant
Applicable Applications for anti-BoTox-E Clone 209F2 antibody
Immunofluorescent Staining of Neuro2A Cells
Application Notes
Immunofluorescent: This monoclonal antibody containing culture supernatant has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure. Following the treatment. the cells were washed x 4. fixed in neutral buffered formalin for 10 min. permeablized and blocked. They were reacted for 60 minutes with the culture supernatant diluted 1:50. and then with FITC-conjugated goat anti-mouse IgG. The immunofluorescent staining pattern was observed using epifluorescent microscopy
Inhibition
Inhibition of BoTox-E entry into Neuro2A Cells
Inhibition Note
This monoclonal antibody containing culture supernatant has been found to inhibit the entry of BoTox-E to Neuro2A cells treated with GT1b ganglioside prior to the addition of the toxin. and prior incubation of the antibody with the synthetic peptide immunogen has been shown to eliminate the inhibition of cellular binding by the antibody
Related Product Information for anti-BoTox-E Clone 209F2 antibody
This sterile filtered culture supernatant contains mouse monoclonal antibody 209F2. a mouse IgG class MAb. raised against a synthetic peptide analogue of the amino terminal region of BoTox-E. This monoclonal antibody has been found to bind to intact whole BoTox-E by ELISA. RIA and cell staining techniques. This monoclonal antibody was tested for recognition of other BoTox isoforms by ELISA and immunocytochemical techniques. Each vial contains 1.0 ml of culture supernatant
Product Categories/Family for anti-BoTox-E Clone 209F2 antibody
Similar Products
Product Notes
The BoTox-E Clone 209F2 (Catalog #AAA350005) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BoTox-E Clone 209F2 can be used in a range of immunoassay formats including, but not limited to, Immunofluorescent Staining of Neuro2A Cells. Immunofluorescent: This monoclonal antibody containing culture supernatant has been found to stain Neuro2A cells treated with GT1b ganglioside prior to the addition of BoTox-E at a dilution of 1:50 using an indirect immunocytochemical staining procedure. Following the treatment. the cells were washed x 4. fixed in neutral buffered formalin for 10 min. permeablized and blocked. They were reacted for 60 minutes with the culture supernatant diluted 1:50. and then with FITC-conjugated goat anti-mouse IgG. The immunofluorescent staining pattern was observed using epifluorescent microscopy. Researchers should empirically determine the suitability of the BoTox-E Clone 209F2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BoTox-E Clone 209F2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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