Rabbit anti-Human DUSP13 Polyclonal Antibody | anti-DUSP13 antibody

DUSP13, CT (DUSP13, BEDP, MDSP, Dual specificity protein phosphatase 13 isoform MDSP, Branching-enzyme interacting DSP, Muscle-restricted DSP) (PE)

Cat. Num. AAA6293795

• Gene Names: DUSP13; BEDP; MDSP; TMDP; SKRP4; DUSP13A; DUSP13B
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DUSP13; Polyclonal Antibody; CT (DUSP13; BEDP; MDSP; Dual specificity protein phosphatase 13 isoform MDSP; Branching-enzyme interacting DSP; Muscle-restricted DSP) (PE); Muscle-restricted DSP; anti-DUSP13 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).

• Applicable Applications for anti-DUSP13 antibody: Western Blot (WB), FLISA
• Application Notes: WB: 1:100-500; FLISA: 1:1,000; Applications are based on unconjugated antibody.
• Immunogen: DUSP13 antibody is generated from rabbits immunized with a KLH conjugated synthetic between 146-173 amino acids selected from the C-terminal region of human DUSP13.
• Conjugate: PE
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(DUSP13 Antibody (C-term) western blot analysis in A2058 cell line lysates (35ug/lane).This demonstrates the DUSP13 antibody detected the DUSP13 protein (arrow).)

Related Product Information for anti-DUSP13 antibody

Members of the protein-tyrosine phosphatase superfamily cooperate with protein kinases to regulate cell proliferation and differentiation. This superfamily is separated into two families based on the substrate that is dephosphorylated. One family, the dual specificity phosphatases (DSPs) acts on both phosphotyrosine and phosphoserine/threonine residues. This gene encodes different but related DSP proteins through the use of non-overlapping open reading frames, alternate splicing, and presumed different transcription promoters. Expression of the distinct proteins from this gene has been found to be tissue specific and the proteins may be involved in postnatal development of specific tissues. A protein encoded by the upstream ORF was found in skeletal muscle, whereas the encoded protein from the downstream ORF was found only in testis. In mouse, a similar pattern of expression was found. Multiple alternatively spliced transcript variants were described, but the full-length sequence of only some were determined.

Product Categories/Family for anti-DUSP13 antibody

Antibodies; Enzymes; Phosphatase

References

Kim, S.J., et al. Proteins 66(1):239-245(2007). Grupe, A., et al. Am. J. Hum. Genet. 78(1):78-88(2006). Barrios-Rodiles, M., et al. Science 307(5715):1621-1625(2005). Chen, H.H., et al. J. Biol. Chem. 279(40):41404-41413(2004). Deloukas, P., et al. Nature 429(6990):375-381(2004).

NCBI and Uniprot Product Information

• NCBI GI #: 56117818
• NCBI GeneID: 51207
• NCBI Accession #: NP_001007272.1
• NCBI GenBank Nucleotide #: NM_001007271.1
• UniProt Accession #: Q6B8I1; Q96J67; Q9UII6
• NCBI Official Full Name: dual specificity protein phosphatase 13 isoform 1
• NCBI Official Synonym Full Names: dual specificity phosphatase 13
• NCBI Official Symbol: DUSP13
• NCBI Official Synonym Symbols: BEDP; MDSP; TMDP; SKRP4; DUSP13A; DUSP13B
• NCBI Protein Information: dual specificity protein phosphatase 13; branching-enzyme interacting DSP; branching-enzyme interacting dual-specificity protein phosphatase; muscle-restricted DSP; testis- and skeletal-muscle-specific DSP
• UniProt Protein Name: Dual specificity protein phosphatase 13 isoform A
• Protein Family: Dual specificity protein phosphatase
• UniProt Gene Name: DUSP13
• UniProt Synonym Gene Names: BEDP; DUSP13A; MDSP; DUSP13A; MDSP
• UniProt Entry Name: DS13A_HUMAN

Uniprot Description

DUSP13 iso5: May be involved in the regulation of meiosis and/or differentiation of testicular germ cells during spermatogenesis. Exhibits intrinsic phosphatase activity towards both phospho- seryl/threonyl and -tyrosyl residues of myelin basic protein, with similar specific activities in vitro. Belongs to the protein-tyrosine phosphatase family. Non-receptor class dual specificity subfamily. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: Cell cycle regulation; EC 3.1.3.16; EC 3.1.3.48; Motility/polarity/chemotaxis; Protein phosphatase, dual-specificity

Chromosomal Location of Human Ortholog: 10q22.2

Molecular Function: phosphoric monoester hydrolase activity; protein binding

Biological Process: dephosphorylation; meiosis; protein amino acid dephosphorylation; spermatogenesis