Mouse BNP PROHORMONE Monoclonal Antibody | anti-NPPB antibody

MOUSE ANTI HUMAN BNP PROHORMONE (N-TERMINAL)

Cat. Num. AAA214420

• Gene Names: NPPB; BNP
• Applications: ELISA, Western Blot
• Synonyms: BNP PROHORMONE; Monoclonal Antibody; MOUSE ANTI HUMAN BNP PROHORMONE (N-TERMINAL); anti-NPPB antibody

• Host: Mouse
• Clonality: Monoclonal
• Isotype: IgG1
• Clone Number: 18H5
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 134

• Applicable Applications for anti-NPPB antibody: ELISA (EIA), Immunoassay (IY), Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 9kDa when using recombinant NT-proBNP.
• Perservative Stabilisers: 0.09% Sodium Azide (NaN3) ; Preparation
• Immunogen: Amino acids 13-27 of NT-proBNP.; Fusion Partners
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Related Product Information for anti-NPPB antibody

Mouse anti Human BNP Prohormone (N-Terminal) antibody, clone 18H5 detects the amino-terminal fragment of pro-brain natriuretic peptide (amino acids 13-20), also known as NT-proBNP, a cleavage product during the proteolytic activation of BNP. NT-proBNP is a secreted protein which is a member of the natriuretic peptide family, which is predominantly secreted from the cardiac ventricles in response to volume and pressure overload. NT-proBNP is more stable than BNP in serum, and has become a useful biomarker of heart failure.

NCBI and Uniprot Product Information

• NCBI GI #: 4505433
• NCBI GeneID: 4879
• NCBI Accession #: NP_002512.1
• NCBI GenBank Nucleotide #: NM_002521.2
• UniProt Accession #: P16860; Q6FGY0; Q9P2Q7; B0ZBE9
• Molecular Weight: 14,726 Da
• NCBI Official Full Name: natriuretic peptides B preproprotein
• NCBI Official Synonym Full Names: natriuretic peptide B
• NCBI Official Symbol: NPPB
• NCBI Official Synonym Symbols: BNP
• NCBI Protein Information: natriuretic peptides B; brain type natriuretic peptide; gamma-brain natriuretic peptide; natriuretic peptide precursor B; natriuretic protein
• UniProt Protein Name: Natriuretic peptides B
• Protein Family: Brain natriuretic peptide
• UniProt Gene Name: NPPB
• UniProt Synonym Gene Names: BNP(1-32); BNP-32
• UniProt Entry Name: ANFB_HUMAN

NCBI Description

This gene is a member of the natriuretic peptide family and encodes a secreted protein which functions as a cardiac hormone. The protein undergoes two cleavage events, one within the cell and a second after secretion into the blood. The protein's biological actions include natriuresis, diuresis, vasorelaxation, inhibition of renin and aldosterone secretion, and a key role in cardiovascular homeostasis. A high concentration of this protein in the bloodstream is indicative of heart failure. The protein also acts as an antimicrobial peptide with antibacterial and antifungal activity. Mutations in this gene have been associated with postmenopausal osteoporosis. [provided by RefSeq, Nov 2014]

Uniprot Description

NPPB: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3. Belongs to the natriuretic peptide family.

Protein type: Hormone; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 1p36.2

Cellular Component: extracellular space; protein complex; extracellular region

Molecular Function: diuretic hormone activity; hormone activity; receptor binding

Biological Process: cGMP biosynthetic process; negative regulation of angiogenesis; cell surface receptor linked signal transduction; regulation of vasodilation; regulation of blood pressure; receptor guanylyl cyclase signaling pathway; negative regulation of cell growth; regulation of vascular permeability; regulation of blood vessel size; body fluid secretion