Rat ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase) ELISA Kit | UCHL1 elisa kit

Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1, UCHL1 ELISA Kit

Cat. Num. AAA932005

• Reactivity: Rat
• Synonyms: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase); Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1; UCHL1 ELISA Kit; Rat Ubiquitin carboxyl-terminal hydrolase isozyme L1 (UCHL1) ELISA kit; PARK5; PGP9.5; PGP95; Uch-L1; neuron cytoplasmic protein 9.5; ubiquitin C-terminal hydrolase; ubiquitin carboxyl-terminal esterase L1; UCHL1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat UCHL1. No significant cross-reactivity or interference between Rat UCHL1 and analogues was observed.
• Sequence Length: 223

• Samples: Serum, plasma, tissue homogenates, Cell lysates
• Assay Type: Sandwich
• Detection Range: 0.78 ng/ml-50 ng/ml
• Sensitivity: 0.2 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Protein Biological Process 1: Ubiquitin
• Protein Biological Process 3: Ubl conjugation pathway
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for UCHL1 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for UCHL1 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any UCHL1 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for UCHL1 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of UCHL1 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 61098212
• NCBI GeneID: 29545
• NCBI Accession #: NP_058933.2
• NCBI GenBank Nucleotide #: NM_017237.3
• UniProt Accession #: Q00981; Q6P9V8
• Molecular Weight: 24,838 Da
• NCBI Official Full Name: ubiquitin carboxyl-terminal hydrolase isozyme L1
• NCBI Official Synonym Full Names: ubiquitin carboxyl-terminal esterase L1 (ubiquitin thiolesterase)
• NCBI Official Symbol: Uchl1
• NCBI Protein Information: ubiquitin carboxyl-terminal hydrolase isozyme L1; PGP9.5; UCH-L1; PGP 9.5; ubiquitin thioesterase L1; neuron cytoplasmic protein 9.5; ubiquitin carboxy-terminal hydrolase L1
• UniProt Protein Name: Ubiquitin carboxyl-terminal hydrolase isozyme L1
• Protein Family: Ubiquitin carboxyl-terminal hydrolase
• UniProt Gene Name: Uchl1
• UniProt Synonym Gene Names: UCH-L1; PGP9.5
• UniProt Entry Name: UCHL1_RAT

NCBI Description

catalyzes the hydrolysis of ubiquitin carboxy-terminal thiolesters to form ubiquitin and a thiol; may play a role in neuropathic pain [RGD, Feb 2006]

Uniprot Description

UCHL1: Ubiquitin-protein hydrolase involved both in the processing of ubiquitin precursors and of ubiquitinated proteins. This enzyme is a thiol protease that recognizes and hydrolyzes a peptide bond at the C-terminal glycine of ubiquitin. Also binds to free monoubiquitin and may prevent its degradation in lysosomes. The homodimer may have ATP-independent ubiquitin ligase activity. Monomer. Homodimer. Interacts with SNCA. Interacts with COPS5. Found in neuronal cell bodies and processes throughout the neocortex. Expressed in neurons and cells of the diffuse neuroendocrine system and their tumors. Weakly expressed in ovary. Down-regulated in brains from Parkinson disease and Alzheimer disease patients. Belongs to the peptidase C12 family.

Protein type: EC 3.4.19.12; Protease; Cell development/differentiation; Ubiquitin conjugating system; Ligase

Cellular Component: nucleoplasm; endoplasmic reticulum membrane; cell soma; cell; axon; cytoplasm; plasma membrane; intracellular; cytosol; nucleus; myelin sheath

Molecular Function: ubiquitin binding; omega peptidase activity; cysteine-type endopeptidase activity; ubiquitin-specific protease activity; alpha-2A adrenergic receptor binding; ligase activity

Biological Process: ubiquitin-dependent protein catabolic process; cell proliferation; negative regulation of MAP kinase activity; axon transport of mitochondrion; protein deubiquitination; axon target recognition; axonogenesis; eating behavior; neuromuscular process; sensory perception of pain; muscle fiber development; adult walking behavior