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Western Blot (WB) (Western blot analysis of BLCAP on recombinant protein tissue lysate using anti-BLCAP antibody at 1/5, 000 dilution.)

Mouse anti-Human, Rat BLCAP Monoclonal Antibody | anti-BLCAP antibody

BLCAP Antibody

Gene Names
BLCAP; BC10
Reactivity
Human, Rat
Applications
Western Blot, Immunohistochemistry, Immunocytochemistry
Purity
ProG affinity purified
Synonyms
BLCAP; Monoclonal Antibody; BLCAP Antibody; Bc10 antibody; Bladder cancer 10 kDa protein antibody; Bladder cancer related protein (10kD) antibody; Bladder cancer-associated protein antibody; BLCAP antibody; BLCAP_HUMAN antibody; anti-BLCAP antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human, Rat
Clonality
Monoclonal
Purity/Purification
ProG affinity purified
Form/Format
1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.
Applicable Applications for anti-BLCAP antibody
Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
Application Notes
WB: 1:1000-1:2000
IHC: 1:50-1:200
ICC: 1:50-1:200
Immunogen
Peptide
Conjugation
Unconjugated
Preparation and Storage
Store at -20 degree C.

Western Blot (WB)

(Western blot analysis of BLCAP on recombinant protein tissue lysate using anti-BLCAP antibody at 1/5, 000 dilution.)

Western Blot (WB) (Western blot analysis of BLCAP on recombinant protein tissue lysate using anti-BLCAP antibody at 1/5, 000 dilution.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-BLCAP antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-BLCAP antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat cervix tissue using anti-BLCAP antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC) (Immunohistochemical analysis of paraffin-embedded rat cervix tissue using anti-BLCAP antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining BLCAP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC) (ICC staining BLCAP (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
Related Product Information for anti-BLCAP antibody
BLCAP is a highly conversed gene with two exons and an intron encoding a 10kDa protein, which was originally identified from invasive bladder carcinoma in 2002. BLCAP is subject to adenosine to inosine (A-to-I) RNA editing. A-to-I RNA editing is an important post-transcription modification of RNA sequence, generating a diversity of RNA products different from the original DNA sequence. The adenosine deaminase acting on double-stranded RNA (ADAR) family of enzymes catalyze the conversion of adenosine (A) to inosine (I) in the double stranded RNA, and inosine is finally recognized as guanosine (G) in the process of mRNA translation. May regulate cell proliferation and coordinate apoptosis and cell cycle progression via a novel mechanism independent of both p53/TP53 and NF-kappa-B.
Product Categories/Family for anti-BLCAP antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
9,876 Da
NCBI Official Full Name
bladder cancer-associated protein
NCBI Official Synonym Full Names
bladder cancer associated protein
NCBI Official Symbol
BLCAP
NCBI Official Synonym Symbols
BC10
NCBI Protein Information
bladder cancer-associated protein; bladder cancer 10 kDa protein; bladder cancer related protein (10kD)
UniProt Protein Name
Bladder cancer-associated protein
UniProt Gene Name
BLCAP
UniProt Synonym Gene Names
BC10; Bc10
UniProt Entry Name
BLCAP_HUMAN

NCBI Description

This gene encodes a tumor suppressor protein that reduces cell growth by stimulating apoptosis. The first intron of this gene contains the neuronatin gene, which is imprinted and only expressed from the paternal allele. This gene is imprinted in brain where it is differentially expressed from different promoters. Transcription from the upstream promoter occurs preferentially on the maternal allele, and transcripts are preferentially expressed from the downstream promoter on the paternal allele. Alternative promoters and alternative splicing result in multiple transcript variants that encode the same protein.[provided by RefSeq, Nov 2009]

Uniprot Description

BLCAP: May regulate cell proliferation and coordinate apoptosis and cell cycle progression via a novel mechanism independent of both p53/TP53 and NF-kappa-B. Belongs to the BLCAP family.

Protein type: Membrane protein, integral; Apoptosis; Membrane protein, multi-pass; Cell cycle regulation

Chromosomal Location of Human Ortholog: 20q11.23

Cellular Component: integral to membrane

Molecular Function: protein binding

Biological Process: apoptotic nuclear changes; cell cycle

Research Articles on BLCAP

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Product Notes

The BLCAP blcap (Catalog #AAA9434998) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BLCAP Antibody reacts with Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's BLCAP can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC). WB: 1:1000-1:2000 IHC: 1:50-1:200 ICC: 1:50-1:200. Researchers should empirically determine the suitability of the BLCAP blcap for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BLCAP, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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