Rabbit DOCK4 Polyclonal Antibody | anti-DOCK4 antibody

DOCK4 Antibody

Cat. Num. AAA9435375

• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunocytochemistry, Immunohistochemistry
• Purity: Peptide affinity purified
• Synonyms: DOCK4; Polyclonal Antibody; DOCK4 Antibody; Dedicator of cytokinesis protein 4 antibody; Dock4 antibody; DOCK4_HUMAN antibody; KIAA0716 antibody; anti-DOCK4 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Purity/Purification: Peptide affinity purified
• Form/Format: 1*TBS (pH7.4), 0.5%BSA, 50%Glycerol. Preservative: 0.05% Sodium Azide.

• Applicable Applications for anti-DOCK4 antibody: Western Blot (WB), Immunocytochemistry (ICC), Immunohistochemistry (IHC)
• Application Notes: IHC: 1:50-1:200; ICC: 1:50-1:200
• Immunogen: Peptide
• Conjugation: Unconjugated
• Preparation and Storage: Store at -20 degree C.

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-DOCK4 antibody. Counter stained with hematoxylin.)

Immunocytochemistry (ICC)

(ICC staining DOCK4 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining DOCK4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Immunocytochemistry (ICC)

(ICC staining DOCK4 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

Related Product Information for anti-DOCK4 antibody

DOCK 4 (Dedicator of cytokinesis protein 4) is a cytoplasmic peripheral membrane protein that belongs to the DOCK family of cytokinesis-regulating proteins. Expressed ubiquitously with highest expression in prostate, ovary and skeletal muscle, DOCK 4 functions as a guanine nucleotide exchange factor (GEF) that activates the small GTPase Rap 1 and, via this activation, plays a role in the regulation of adherens junctions between cells. Similar to other DOCK family members, DOCK 4 contains an N-terminal SH3 domain, a C-terminal proline-rich region and two internal DOCK homology regions designated DHR1 and DHR2. Defects in the gene encoding DOCK 4 result in the inactivation of Rap 1 and are, thus, implicated in the pathogenesis of various cancers such as ovarian, prostate, glioma and colorectal carcinomas. Four isoforms of DOCK 4 are expressed due to alternative splicing events.

Product Categories/Family for anti-DOCK4 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 92091572
• NCBI GeneID: 9732
• NCBI Accession #: NP_055520.3
• NCBI GenBank Nucleotide #: NM_014705.3
• UniProt Accession #: Q8N1I0; O14584; O94824; Q8NB45
• Molecular Weight: 29,833 Da
• NCBI Official Full Name: dedicator of cytokinesis protein 4
• NCBI Official Synonym Full Names: dedicator of cytokinesis 4
• NCBI Official Symbol: DOCK4
• NCBI Protein Information: dedicator of cytokinesis protein 4
• UniProt Protein Name: Dedicator of cytokinesis protein 4
• Protein Family: Dedicator of cytokinesis protein
• UniProt Gene Name: DOCK4
• UniProt Synonym Gene Names: KIAA0716

NCBI Description

This gene is a member of the dedicator of cytokinesis (DOCK) family and encodes a protein with a DHR-1 (CZH-1) domain, a DHR-2 (CZH-2) domain and an SH3 domain. This membrane-associated, cytoplasmic protein functions as a guanine nucleotide exchange factor and is involved in regulation of adherens junctions between cells. Mutations in this gene have been associated with ovarian, prostate, glioma, and colorectal cancers. Alternatively spliced variants which encode different protein isoforms have been described, but only one has been fully characterized. [provided by RefSeq, Jul 2008]

Uniprot Description

Involved in regulation of adherens junction between cells. Plays a role in cell migration. Functions as a guanine nucleotide exchange factor (GEF), which activates Rap1 small GTPase by exchanging bound GDP for free GTP.