IHC
(Biotinylated STAT5 antibody was detected by MsxBiotin at 1:100, and visualized by FITC-Goat anti Ms IgG. The STAT5 is nuclear located.)
Mouse anti-All species Biotin Monoclonal Antibody | anti-Biotin antibody
Mouse anti Biotin Monoclonal antibody
Reactivity
All species
Applications
ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation
Purity
The mouse IgG1 is purified by Protein A-Affinity Chromatography according to Isotyping
Synonyms
Biotin; Monoclonal Antibody; Mouse anti Biotin Monoclonal antibody; Mouse anti-Biotin; N/A; anti-Biotin antibody
Host
Mouse
Reactivity
All species
Clonality
Monoclonal
Isotype
IgG1, k
Clone Number
SPM375
Specificity
This antibody recognizes free Biotin or bound-form biotin, including protein-Biotin, DNA/RNA-Biotin.
Purity/Purification
The mouse IgG1 is purified by Protein A-Affinity Chromatography according to Isotyping
Form/Format
This affinity purified antibody is supplied in sterile Phosphate-buffered saline (pH7.2) containing antibody stabilizer
Applicable Applications for anti-Biotin antibody
ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, In Situ Nucleic Acid hybridization
Application Notes
Western Blot: 0.5-2 ug/ml
ELISA: 0.1-1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100
In Situ Nucleic Acid Hybridization: 2-5 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
ELISA: 0.1-1 ug/ml
Immunoprecipitation: 2-5 ug/ml
Immunohistochemistry: 1:100
In Situ Nucleic Acid Hybridization: 2-5 ug/ml
Optimal dilutions should be determined by researchers for the specific applications.
Antigen Preparation
Biotin-conjugates as an immunogen.
Positive Control
Free Biotin or Bound-form Biotin
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20 degree C to -70 degree C. The antibodies can be stored at 2 degree C-8 degree C for three month without detectable loss of activity. Avoid repeated freezing-thawing cycles.
IHC
(Biotinylated STAT5 antibody was detected by MsxBiotin at 1:100, and visualized by FITC-Goat anti Ms IgG. The STAT5 is nuclear located.)
IHC
(Biotinylated STAT5 antibody was detected by MsxBiotin at 1:100, and visualized by FITC-Goat anti Ms IgG. The STAT5 is nuclear located.)
Related Product Information for anti-Biotin antibody
This antibody has Ab-1 has a very high affinity for biotin and is excellent for use in various amplification techniques. It recognizes both the free and protein conjugated (either soluble or cell bound) form of biotin. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
References
Elisabeth D. Coene et al (2008). Anti-Biotin Antibodies Offer Superior Organelle-Specific Labelling of Mitochondria Over Avidin or Streptavidin. Methods in Molecular Biology, Volume 418. 157-170.
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Product Notes
The Biotin (Catalog #AAA462194) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti Biotin Monoclonal antibody reacts with All species and may cross-react with other species as described in the data sheet. AAA Biotech's Biotin can be used in a range of immunoassay formats including, but not limited to, ELISA, Western Blot, Immunohistochemistry, Immunoprecipitation, In Situ Nucleic Acid hybridization. Western Blot: 0.5-2 ug/ml ELISA: 0.1-1 ug/ml Immunoprecipitation: 2-5 ug/ml Immunohistochemistry: 1:100 In Situ Nucleic Acid Hybridization: 2-5 ug/ml Optimal dilutions should be determined by researchers for the specific applications. Researchers should empirically determine the suitability of the Biotin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Biotin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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