FCM/FACS (Flow Cytometry)
(Separation of human CD75 positive lymphocytes (red-filled) from CD75 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD75 (LN1) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
anti-Human CD75 Antibody | anti-CD75 antibody
Anti-Hu CD75 PE
Reactivity
Human
Applications
Flow Cytometry, Functional Assay
Synonyms
CD75; N/A; Anti-Hu CD75 PE; lactosamine; anti-CD75 antibody
Reactivity
Human
Isotype
Mouse IgM kappa
Clone Number
LN1
Specificity
The mouse monoclonal antibody LN1 recognizes CD75, a lactosamine structure present mainly on the surface of B cell types.
Form/Format
PE
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Stabilizing Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide
Applicable Applications for anti-CD75 antibody
FCM/FACS (Flow Cytometry)
Immunogen
Stimulated human PBL
Preparation and Storage
Store at 2-8 degree C. Protect from prolonged exposure to light. Do not freeze.
FCM/FACS (Flow Cytometry)
(Separation of human CD75 positive lymphocytes (red-filled) from CD75 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD75 (LN1) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Separation of human CD75 positive lymphocytes (red-filled) from CD75 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD75 (LN1) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD75 (LN1) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
FCM/FACS (Flow Cytometry)
(Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD75 (LN1) PE antibody (10 ?l reagent / 100 ?l of peripheral whole blood).)
Related Product Information for anti-CD75 antibody
CD75 is a lactosamine structure, which is present mainly on the surface of germinal center B cells. With lower level it is present on other mature B cells, and it is downregulated during differentiation to plasma cells. It is a marker which differentiates between malignant B cell types.
References
*Fromm JR, Thomas A, Wood BL: Characterization and purification of neoplastic cells of nodular lymphocyte predominant hodgkin lymphoma from lymph nodes by flow cytometry and flow cytometric cell sorting. Am J Pathol. 2017 Feb;187(2):304-317., URL: https://pubmed.ncbi.nlm.nih.gov/27998726/,*Epstein AL, Marder RJ, Winter JN, Fox RI: Two new monoclonal antibodies (LN-1, LN-2) reactive in B5 formalin-fixed, paraffin-embedded tissues with follicular center and mantle zone human B lymphocytes and derived tumors. J Immunol. 1984 Aug;133(2):1028-36., URL: https://pubmed.ncbi.nlm.nih.gov/6376628/,*Virtanen S, Schulte R, Stingl J, Caldas C, Shehata M: High-throughput surface marker screen on primary human breast tissues reveals further cellular heterogeneity. Breast Cancer Res. 2021 Jun 13;23(1):66., URL: https://pubmed.ncbi.nlm.nih.gov/34120626/,
Similar Products
Product Notes
The CD75 (Catalog #AAA129232) is an Antibody and is intended for research purposes only. The product is available for immediate purchase. The Anti-Hu CD75 PE reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's CD75 can be used in a range of immunoassay formats including, but not limited to, FCM/FACS (Flow Cytometry). Researchers should empirically determine the suitability of the CD75 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "CD75, Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.