Western Blot (WB)
(Western Blot: The biotinylated IgG was resolved onto 12% SDS-PAGE, transferred onto NC membrane, and detected by Mouse anti-Biotin (Cat# 604-100) at 1:1000. Observed two major immunoreactive bands at molecular weight 50kDa and 25 kDa.)
Mouse Biotin Monoclonal Antibody
Mouse anti Biotin Monoclonal Antibody
Applications
Western Blot, ELISA, Immunoprecipitation, Immunohistochemistry
Purity
The Mouse IgG is purified by Affinity Purification
Synonyms
Biotin; Monoclonal Antibody; Mouse anti Biotin Monoclonal Antibody; anti-Biotin antibody
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Specificity
This antibody recognizes biotin compound.
Purity/Purification
The Mouse IgG is purified by Affinity Purification
Form/Format
This affinity purified antibody is supplied in sterile phosphate buffered saline (pH 7.2) containing antibody stabilizer.
Sequence Length
320
Applicable Applications for anti-Biotin antibody
Western Blot, ELISA, Immunoprecipitation, IHC, In Situ Nucleic Acid Hybridization
Application Notes
Western Blot: 0.1-1 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
IHC: 2-5 ug/ml
ELISA: 0.01-0.1 ug/ml
Immunoprecipitation: 2-5 ug/ml
IHC: 2-5 ug/ml
Antigen Preparation
A chemical linked Biotin
Positive Control
Biotinylated protein
Preparation and Storage
The antibodies are stable for 12 months from date of receipt when stored at -20°C to -70°C. The antibodies can be stored at 2° to 8°C for three months without detectable loss of activity. Avoid repeated freezing-thawing cycles.
Western Blot (WB)
(Western Blot: The biotinylated IgG was resolved onto 12% SDS-PAGE, transferred onto NC membrane, and detected by Mouse anti-Biotin (Cat# 604-100) at 1:1000. Observed two major immunoreactive bands at molecular weight 50kDa and 25 kDa.)
Western Blot (WB)
(Western Blot: The biotinylated IgG was resolved onto 12% SDS-PAGE, transferred onto NC membrane, and detected by Mouse anti-Biotin (Cat# 604-100) at 1:1000. Observed two major immunoreactive bands at molecular weight 50kDa and 25 kDa.)
Related Product Information for anti-Biotin antibody
Biotin is a water-soluble vitamin that serves as an essential coenzyme for five carboxylases in mammals. Biotin-dependent carboxylases catalyze the fixation of bicarbonate in organic acids and play crucial roles in the metabolism of fatty acids, amino acids and glucose. Carboxylase activities decrease substantially in response to biotin deficiency. Biotin is also covalently attached to histones; biotinylated histones are enriched in repeat regions in the human genome and appear to play a role in transcriptional repression of genes and genome stability. In some applications, localization of biotinylated probes with avidin produces unacceptably high background staining. Anti-biotin antibody may be substituted to decrease this noise.
NCBI and Uniprot Product Information
NCBI GI #
UniProt Accession #
Molecular Weight
244 Dalton (plus biotinylated protein)
NCBI Official Full Name
biotin
Protein Family
Similar Products
Product Notes
The Biotin (Catalog #AAA460481) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's Biotin can be used in a range of immunoassay formats including, but not limited to, Western Blot, ELISA, Immunoprecipitation, IHC, In Situ Nucleic Acid Hybridization. Western Blot: 0.1-1 ug/ml ELISA: 0.01-0.1 ug/ml Immunoprecipitation: 2-5 ug/ml IHC: 2-5 ug/ml. Researchers should empirically determine the suitability of the Biotin for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Biotin, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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