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Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BDH2 protein (Right) or empty vector (Left) detected with BDH2 antibody)

Mouse BDH2 Monoclonal Antibody | anti-BDH2 antibody

BDH2 antibody

Gene Names
BDH2; DHRS6; EFA6R; SDR15C1; UCPA-OR; UNQ6308; PRO20933
Applications
Flow Cytometry, Functional Assay, Immunofluorescence, Immunohistochemistry
Purity
BDH2 antibody was purified by affinity chromatography.
Synonyms
BDH2; Monoclonal Antibody; BDH2 antibody; Monoclonal BDH2; Anti-BDH2; 3-Hydroxybutyrate Dehydrogenase Type 2; DHRS6; EFA6R; FLJ13261; PRO20933; UCPA-OR; UNQ6308; BDH 2; BDH-2; anti-BDH2 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1
Clone Number
3G10
Purity/Purification
BDH2 antibody was purified by affinity chromatography.
Form/Format
Supplied in PBS buffer, pH 7.3, containing 1% BSA, 50% glycerol and 0.02% sodium azide.
Concentration
500 ug-1 mg/ml (varies by lot)
Sequence Length
245
Applicable Applications for anti-BDH2 antibody
Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC)
Application Notes
FC: 1:100, IF: 1:100, IHC: 1:50
Biological Significance
BDH2 is a dehydrogenase that mediates the formation of 2, 5-dihydroxybenzoic acid (2, 5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. It also acts as a 3-hydroxybutyrate dehydrogenase.
Cross-Reactivity
Human
Immunogen
BDH2 antibody was raised in mouse using a full length recombinant protein of human BDH2 (NP_064524) produced in HEK293T cells, as the immunogen.
Preparation and Storage
Store at 4 degree C for short term storage. Aliquot and store at -20 degree C for long term storage. Avoid repeated freeze/thaw cycles.

Western Blot (WB)

(Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BDH2 protein (Right) or empty vector (Left) detected with BDH2 antibody)

Western Blot (WB) (Western Blot analysis of HEK293T cell lysates (5 ug) transfected with either recombinant BDH2 protein (Right) or empty vector (Left) detected with BDH2 antibody)

Immunohistochemistry (IHC)

(Immunohistochemical analysis of BDH2 protein in paraffin embedded Human pancreas tissue using BDH2 antibody)

Immunohistochemistry (IHC) (Immunohistochemical analysis of BDH2 protein in paraffin embedded Human pancreas tissue using BDH2 antibody)

Immunofluorescence (IF)

(Immunofluorescent staining of COS7 cells transiently transfected with recombinant BDH2 protein using BDH2 antibody)

Immunofluorescence (IF) (Immunofluorescent staining of COS7 cells transiently transfected with recombinant BDH2 protein using BDH2 antibody)
Related Product Information for anti-BDH2 antibody
Mouse monoclonal BDH2 antibody
Product Categories/Family for anti-BDH2 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15,626 Da
NCBI Official Full Name
3-hydroxybutyrate dehydrogenase type 2
NCBI Official Synonym Full Names
3-hydroxybutyrate dehydrogenase, type 2
NCBI Official Symbol
BDH2
NCBI Official Synonym Symbols
DHRS6; EFA6R; SDR15C1; UCPA-OR; UNQ6308; PRO20933
NCBI Protein Information
3-hydroxybutyrate dehydrogenase type 2
UniProt Protein Name
3-hydroxybutyrate dehydrogenase type 2
UniProt Gene Name
BDH2
UniProt Synonym Gene Names
DHRS6; SDR15C1
UniProt Entry Name
BDH2_HUMAN

Uniprot Description

DHRS6: Dehydrogenase that mediates the formation of 2,5- dihydroxybenzoic acid (2,5-DHBA), a siderophore that shares structural similarities with bacterial enterobactin and associates with LCN2, thereby playing a key role in iron homeostasis and transport. Also acts as a 3-hydroxybutyrate dehydrogenase. Belongs to the short-chain dehydrogenases/reductases (SDR) family. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Lipid Metabolism - synthesis and degradation of ketone bodies; Oxidoreductase; Carbohydrate Metabolism - butanoate; EC 1.1.1.30

Chromosomal Location of Human Ortholog: 4q24

Cellular Component: mitochondrion; cytoplasm

Molecular Function: oxidoreductase activity, acting on the CH-CH group of donors, NAD or NADP as acceptor; 3-hydroxybutyrate dehydrogenase activity; NAD binding

Biological Process: iron ion homeostasis; siderophore biosynthetic process; fatty acid beta-oxidation; epithelial cell differentiation; heme metabolic process

Research Articles on BDH2

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Product Notes

The BDH2 bdh2 (Catalog #AAA831576) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BDH2 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF), Immunohistochemistry (IHC). FC: 1:100, IF: 1:100, IHC: 1:50. Researchers should empirically determine the suitability of the BDH2 bdh2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BDH2, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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