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Western Blot (WB) (Western blot analysis of SNRPD2 Antibody (N-term) in Hela cell line lysates (35ug/lane). SNRPD2 (arrow) was detected using the purified Pab.)

Rabbit anti-Human SNRPD2 Polyclonal Antibody | anti-SNRPD2 antibody

SNRPD2, NT (Small Nuclear Ribonucleoprotein Sm D2, Sm-D2, snRNP Core Protein D2, SNRPD1) (FITC)

Gene Names
SNRPD2; SMD2; Sm-D2; SNRPD1
Reactivity
Human
Applications
Western Blot, ELISA
Purity
Purified by Ammonium Sulfate Precipitation.
Synonyms
SNRPD2; Polyclonal Antibody; NT (Small Nuclear Ribonucleoprotein Sm D2; Sm-D2; snRNP Core Protein D2; SNRPD1) (FITC); anti-SNRPD2 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes human SNRPD2.
Purity/Purification
Purified by Ammonium Sulfate Precipitation.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).
Applicable Applications for anti-SNRPD2 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB: 1:50-1:100
ELISA: 1:1,000
Applications are based on unconjugated antibody.
Immunogen
Synthetic peptide selected from the N-terminal region of human SNRPD2 (KLH).
Conjugate
FITC
Note
Preservative Free
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer.

Western Blot (WB)

(Western blot analysis of SNRPD2 Antibody (N-term) in Hela cell line lysates (35ug/lane). SNRPD2 (arrow) was detected using the purified Pab.)

Western Blot (WB) (Western blot analysis of SNRPD2 Antibody (N-term) in Hela cell line lysates (35ug/lane). SNRPD2 (arrow) was detected using the purified Pab.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SNRPD2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Immunohistochemistry (IHC) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SNRPD2 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

Flow Cytometry (FC/FACS)

(SNRPD2 Antibody (N-term) flow cytometry analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Flow Cytometry (FC/FACS) (SNRPD2 Antibody (N-term) flow cytometry analysis of NCI-H292 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)
Product Categories/Family for anti-SNRPD2 antibody
References
1. Stelzl, U., et.al., Cell 122(6), 957-968 (2005).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
15.6 kDa (138aa), confirmed by MALDI-TOF
NCBI Official Full Name
small nuclear ribonucleoprotein Sm D2 isoform 1
NCBI Official Synonym Full Names
small nuclear ribonucleoprotein D2 polypeptide
NCBI Official Symbol
SNRPD2
NCBI Official Synonym Symbols
SMD2; Sm-D2; SNRPD1
NCBI Protein Information
small nuclear ribonucleoprotein Sm D2
UniProt Protein Name
Small nuclear ribonucleoprotein Sm D2
UniProt Gene Name
SNRPD2
UniProt Synonym Gene Names
SNRPD1; Sm-D2
UniProt Entry Name
SMD2_HUMAN

NCBI Description

The protein encoded by this gene belongs to the small nuclear ribonucleoprotein core protein family. It is required for pre-mRNA splicing and small nuclear ribonucleoprotein biogenesis. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, May 2009]

Uniprot Description

snRNP D2: Required for pre-mRNA splicing. Required for snRNP biogenesis. Belongs to the snRNP core protein family.

Protein type: RNA splicing; Spliceosome

Chromosomal Location of Human Ortholog: 19q13.2

Cellular Component: small nuclear ribonucleoprotein complex; nucleoplasm; spliceosome; snRNP U1; cytosol; U12-dependent spliceosome

Molecular Function: protein binding

Biological Process: nuclear mRNA splicing, via spliceosome; spliceosomal snRNP biogenesis; RNA splicing; spliceosome assembly; gene expression

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Product Notes

The SNRPD2 snrpd2 (Catalog #AAA6500032) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The SNRPD2, NT (Small Nuclear Ribonucleoprotein Sm D2, Sm-D2, snRNP Core Protein D2, SNRPD1) (FITC) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's SNRPD2 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB: 1:50-1:100 ELISA: 1:1,000 Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the SNRPD2 snrpd2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "SNRPD2, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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