anti-Human AFP Polyclonal Antibody | anti-AFP antibody

Anti-AFP Polyclonal Antibody

Cat. Num. AAA847591

• Gene Names: AFP; FETA; HPAFP
• Reactivity: Human
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Affinity purification
• Synonyms: AFP; Polyclonal Antibody; Anti-AFP Polyclonal Antibody; AFPD; Alpha fetoglobulin; Alpha fetoprotein; Alpha fetoprotein precursor; Alpha-1-fetoprotein; FETA; anti-AFP antibody

• Host: Human, Mouse chimeric
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Polyclonal antibody to detect AFP
• Purity/Purification: Affinity purification
• Form/Format: Colorless liquid. PBS with 0.05% sodium azide, 50% glycerol, pH7.3
• Concentration: 0.5mg/ml (varies by lot)

• Applicable Applications for anti-AFP antibody: Immunohistochemistry (IHC), Western Blot (WB), ELISA (EIA)
• Application Notes: WB: 1:1000-1:5000; IHC: 1:50-1:200
• Immunogen: Recombinant protein of human AFP
• Preparation and Storage: Store at 4 degree C; The antibody solution should be gently mixed before use.; Shelf life: ~12 months

Immunohistochemistry (IHC)

Western Blot (WB)

Related Product Information for anti-AFP antibody

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly.

Product Categories/Family for anti-AFP antibody

Tumor Biomarker; Antibodies & Supporting Tools; Biomarker Assay Antibodies; AFP

NCBI and Uniprot Product Information

• NCBI GI #: 4501989
• NCBI GeneID: 174
• NCBI Accession #: NP_001125.1
• NCBI GenBank Nucleotide #: NM_001134.1
• UniProt Accession #: P02771; B2RBU3
• Molecular Weight: 68,678 Da
• NCBI Official Full Name: alpha-fetoprotein
• NCBI Official Synonym Full Names: alpha-fetoprotein
• NCBI Official Symbol: AFP
• NCBI Official Synonym Symbols: FETA; HPAFP
• NCBI Protein Information: alpha-fetoprotein; alpha-fetoglobulin; alpha-1-fetoprotein
• UniProt Protein Name: Alpha-fetoprotein
• Protein Family: AFP
• UniProt Gene Name: AFP
• UniProt Synonym Gene Names: HPAFP
• UniProt Entry Name: FETA_HUMAN

NCBI Description

This gene encodes alpha-fetoprotein, a major plasma protein produced by the yolk sac and the liver during fetal life. Alpha-fetoprotein expression in adults is often associated with hepatoma or teratoma. However, hereditary persistance of alpha-fetoprotein may also be found in individuals with no obvious pathology. The protein is thought to be the fetal counterpart of serum albumin, and the alpha-fetoprotein and albumin genes are present in tandem in the same transcriptional orientation on chromosome 4. Alpha-fetoprotein is found in monomeric as well as dimeric and trimeric forms, and binds copper, nickel, fatty acids and bilirubin. The level of alpha-fetoprotein in amniotic fluid is used to measure renal loss of protein to screen for spina bifida and anencephaly. [provided by RefSeq, Jul 2008]

Uniprot Description

Function: Binds copper, nickel, and fatty acids as well as, and bilirubin less well than, serum albumin. Only a small percentage (less than 2%) of the human AFP shows estrogen-binding properties.

Subunit structure: Dimeric and trimeric forms have been found in addition to the monomeric form.

Subcellular location: Secreted.

Tissue specificity: Plasma. Synthesized by the fetal liver and yolk sac.

Developmental stage: Occurs in the plasma of fetuses more than 4 weeks old, reaches the highest levels during the 12th-16th week of gestation, and drops to trace amounts after birth. The serum level in adults is usually less than 40 ng/ml. AFP occurs also at high levels in the plasma and ascitic fluid of adults with hepatoma.

Post-translational modification: Independent studies suggest heterogeneity of the N-terminal sequence of the mature protein and of the cleavage site of the signal sequence.Sulfated.

Sequence similarities: Belongs to the ALB/AFP/VDB family.Contains 3 albumin domains.