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Western Blot (WB) (Western Blot detection against Immunogen (51.37kD).)

Mouse anti-Human BCL2L1 Monoclonal Antibody | anti-BCL2L1 antibody

BCL2L1 (Bcl-2-like Protein 1, Bcl2-L-1, Apoptosis Regulator Bcl-X, BCL2L, BCLX) (PE)

Gene Names
BCL2L1; BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
Reactivity
Human
Applications
ELISA, Immunofluorescence, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
BCL2L1; Monoclonal Antibody; BCL2L1 (Bcl-2-like Protein 1; Bcl2-L-1; Apoptosis Regulator Bcl-X; BCL2L; BCLX) (PE); anti-BCL2L1 antibody
Ordering
For Research Use Only!
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
3E2-2A5
Specificity
Recognizes human BCL2L1.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with R-Phycoerythrin (PE).
Sequence Length
2575
Applicable Applications for anti-BCL2L1 antibody
ELISA (EIA), Immunofluorescence (IF), Western Blot (WB)
Application Notes
IF: 10ug/ml
Applications are based on unconjugated antibody.
Immunogen
Full length recombinant corresponding to aa1-233 from human BCL2L1 (AAH19307) with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
MSQSNRELVVDFLSYKLSQKGYSWSQFSDVEENRTEAPEGTESEMETPSAINGNPSWHLADSPAVNGATGHSSSLDAREVIPMAAVKQALREAGDEFELRYRRAFSDLTSQLHITPGTAYQSFEQVVNELFRDGVNWGRIVAFFSFGGALCVESVDKEMQVLVSRIAAWMATYLNDHLEPWIQENGGWDTFVELYGNNAAAESRKGQERFNRWFLTGMTVAGVVLLGSLFSRK
Conjugate
PE
Preparation and Storage
Store product at 4 degree C in the dark. DO NOT FREEZE! Stable at 4 degree C for 12 months after receipt as an undiluted liquid. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: PE conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (51.37kD).)

Western Blot (WB) (Western Blot detection against Immunogen (51.37kD).)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to BCL2L1 on A-431 cell. [antibody concentration 10ug/ml].)

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to BCL2L1 on A-431 cell. [antibody concentration 10ug/ml].)

Testing Data

(Detection limit for recombinant GST tagged BCL2L1 is ~0.03ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged BCL2L1 is ~0.03ng/ml as a capture antibody.)

Testing Data

(Proximity Ligation Analysis (PLA) of protein-protein interactions between BID and BCL2L1. HeLa cells were stained with BID rabbit purified polyclonal 1:1200 and BCL2L1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Testing Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between BID and BCL2L1. HeLa cells were stained with BID rabbit purified polyclonal 1:1200 and BCL2L1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Western Blot (WB)

(Western Blot analysis of BCL2L1 expression in transfected 293T cell line by BCL2L1 monoclonal antibody. Lane 1: BCL2L1 transfected lysate (26kD). Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of BCL2L1 expression in transfected 293T cell line by BCL2L1 monoclonal antibody. Lane 1: BCL2L1 transfected lysate (26kD). Lane 2: Non-transfected lysate.)
Product Categories/Family for anti-BCL2L1 antibody
References
1. A BACH2/BCL2L1 fusion gene resulting from at (6; 20)(q15; q11. 2) chromosomal translocation in the lymphoma cell line BLUE-1. Turkmen S, Riehn M, Klopocki E, Molkentin M, Reinhardt R, Burmeister T.Genes Chromosomes Cancer. 2011 Jun;50(6):389-96. doi: 10.1002/gcc.20863. Epub 2011 Mar 15.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
598
NCBI Official Full Name
Homo sapiens BCL2-like 1, mRNA
NCBI Official Synonym Full Names
BCL2 like 1
NCBI Official Symbol
BCL2L1
NCBI Official Synonym Symbols
BCLX; BCL2L; Bcl-X; PPP1R52; BCL-XL/S
NCBI Protein Information
bcl-2-like protein 1
Protein Family

NCBI Description

The protein encoded by this gene belongs to the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. The proteins encoded by this gene are located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential, and thus controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Alternative splicing results in multiple transcript variants encoding two different isoforms. The longer isoform acts as an apoptotic inhibitor and the shorter isoform acts as an apoptotic activator. [provided by RefSeq, Dec 2015]

Research Articles on BCL2L1

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Product Notes

The BCL2L1 (Catalog #AAA6156714) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The BCL2L1 (Bcl-2-like Protein 1, Bcl2-L-1, Apoptosis Regulator Bcl-X, BCL2L, BCLX) (PE) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BCL2L1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Immunofluorescence (IF), Western Blot (WB). IF: 10ug/ml Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BCL2L1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BCL2L1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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