Mouse anti-Human ACP2 / Acid Phosphatase 2 Monoclonal Antibody | anti-ACP2 antibody

Mouse Monoclonal [clone M1-4A12] (IgG2b,l) to Human ACP2 / Acid Phosphatase 2

Cat. Num. AAA246116

• Gene Names: ACP2; LAP
• Reactivity: Human
• Applications: Immunohistochemistry, Immunofluorescence, Western Blot, ELISA
• Purity: Protein A Purified
• Synonyms: ACP2 / Acid Phosphatase 2; Monoclonal Antibody; Mouse Monoclonal [clone M1-4A12] (IgG2b; l) to Human ACP2 / Acid Phosphatase 2; Anti-ACP2 / Acid Phosphatase 2 Antibody (clone M1-4A12) IHC-plus; ACP2; Acid Phosphatase 2; Acid phosphatase 2; lysosomal; LAP; Lysosomal acid phosphatase; Human ACP2; anti-ACP2 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2b,l
• Clone Number: M1-4A12
• Specificity: Human ACP2
• Purity/Purification: Protein A Purified
• Form/Format: PBS, pH 7.2
• Concentration: 0.41 mg/ml (varies by lot)
• Sequence Length: 423

• Applicable Applications for anti-ACP2 antibody: Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IF (10 ug/ml), IHC-P (10 ug/ml), WB; Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections. RNAi Knockdown: Antibody validated. Western Blot: Transfected lysate, Recombinant protein.
• Target Species: Human
• Immunogen Description: ACP2 (AAH03160, 1 a.a. ~ 424 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Immunogen: ACP2 / Acid Phosphatase 2 antibody was raised against aCP2 (AAH03160, 1 a.a. ~ 424 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Preparation and Storage: Short term 4 degree C, long term aliquot and store at -20 degree C, avoid freeze thaw cycles.

Immunohistochemistry (IHC)

(Anti-ACP2 / Acid Phosphatase 2 antibody IHC of human kidney, tubules. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)

Immunofluorescence (IF)

(Immunofluorescence of monoclonal antibody to ACP2 on HeLa cell. [antibody concentration 10 ug/ml])

Western Blot (WB)

(Western blot of ACP2 expression in transfected 293T cell line by ACP2 monoclonal antibody (M01), clone M1-4A12.)

RNA interference

(Western blot of ACP2 over-expressed 293 cell line, cotransfected with ACP2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ACP2 monoclonal antibody, clone M1-4A12. GAPDH (36.1 kD) used as specificity and loading control.)

Related Product Information for anti-ACP2 antibody

Lysosomal acid phosphatase is comprised of two subunits, alpha and beta, and is chemically and genetically distinct from red cell acid phosphatase. Lysosomal acid phosphatase 2 is a member of a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. Acid phosphatase deficiency is caused by mutations in the ACP2 (beta subunit) and ACP3 (alpha subunit) genes.

NCBI and Uniprot Product Information

• NCBI GI #: 4557010
• NCBI GeneID: 53
• NCBI Accession #: NP_001601.1
• NCBI GenBank Nucleotide #: NM_001610.3
• UniProt Accession #: P11117; Q561W5; Q9BTU7; E9PCI1
• Molecular Weight: 18,417 Da
• NCBI Official Full Name: lysosomal acid phosphatase isoform 1
• NCBI Official Synonym Full Names: acid phosphatase 2, lysosomal
• NCBI Official Symbol: ACP2
• NCBI Official Synonym Symbols: LAP
• NCBI Protein Information: lysosomal acid phosphatase
• UniProt Protein Name: Lysosomal acid phosphatase
• Protein Family: Cysteine proteinase
• UniProt Gene Name: ACP2
• UniProt Synonym Gene Names: LAP
• UniProt Entry Name: PPAL_HUMAN

NCBI Description

This gene encodes the beta subunit of lysosomal acid phosphatase (LAP). LAP is chemically and genetically distinct from red cell acid phosphatase. The encoded protein belongs to a family of distinct isoenzymes which hydrolyze orthophosphoric monoesters to alcohol and phosphate. LAP-deficiencies in mice cause multiple defects including bone structure alterations, lysosomal storage defects in the kidneys and central nervous system, and an increased tendency towards seizures. An enzymatically-inactive allele of LAP in mice exhibited a more severe phenotype than the null allele, and defects included cerebellum abnormalities, growth retardation, hair-follicle abnormalities, and an ataxia-like phenotype. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq, Oct 2014]

Uniprot Description

ACP2: Defects in ACP2 are a cause of acid phosphatase deficiency (ACPHD). The clinical features are intermittent vomiting, hypotonia, lethargy, opisthotonos, terminal bleeding, and death in early infancy. Lysosomal acid phosphatase is deficient in cultured fibroblasts and multiple tissues. Belongs to the histidine acid phosphatase family.

Protein type: EC 3.1.3.2; Cofactor and Vitamin Metabolism - riboflavin; Motility/polarity/chemotaxis; Phosphatase (non-protein); Membrane protein, integral

Chromosomal Location of Human Ortholog: 11p11.2|11p12-p11

Cellular Component: lysosomal lumen; membrane; lysosome; lysosomal membrane; integral to membrane

Molecular Function: acid phosphatase activity

Biological Process: dephosphorylation; lysosome organization and biogenesis; skeletal development

Disease: Acid Phosphatase Deficiency