Immunohistochemistry (IHC)
(Immunohistochemical staining of GAD in rat hippocampus using MBS607899.)
Mouse anti-Rat Glutamic Acid Decarboxylase Monoclonal Antibody
Glutamic Acid Decarboxylase (GAD)
Reactivity
Rat
Applications
Western Blot, Immunohistochemistry
Purity
PurifiedPurified immunoglobulin
Synonyms
Glutamic Acid Decarboxylase; Monoclonal Antibody; Glutamic Acid Decarboxylase (GAD); Anti -Glutamic Acid Decarboxylase (GAD); anti-Glutamic Acid Decarboxylase antibody
Host
Mouse
Reactivity
Rat
Clonality
Monoclonal
Isotype
IgG2a
Clone Number
2Q61
Specificity
Recognizes the lower MW isoform (65kDa) of the two GAD isoforms identified in brain. This monclonal antibody can be used for immunohistochemical localization in brain or pancreas. Has been used to label purified GAD on Western blots.
Purity/Purification
Purified
Purified immunoglobulin
Purified immunoglobulin
Form/Format
Supplied as a lyophilized powder from PBS, pH 7.4. Reconstitute with 100ul sterile 40-50% glycerol, ddH2O.
Applicable Applications for anti-Glutamic Acid Decarboxylase antibody
Western Blot (WB), Immunohistochemistry (IHC)
Application Notes
Suitable for use in Western Blot and Immunohistochemistry.
Dilution: Immunohistochemistry: 1ug/ml
Dilution: Immunohistochemistry: 1ug/ml
Immunogen
Purified rat brain glutamic acid decarboxylase (GAD).
Preparation and Storage
Lyophilized powder may be stored at 4 degree C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol, ddH2O and store at -20 degree C. Reconstituted product is stable for 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Immunohistochemistry (IHC)
(Immunohistochemical staining of GAD in rat hippocampus using MBS607899.)
Immunohistochemistry (IHC)
(Immunohistochemical staining of GAD in rat hippocampus using MBS607899.)
Related Product Information for anti-Glutamic Acid Decarboxylase antibody
The enzyme glutamic acid decarboxylase (GAD) catalyzes the synthesis of g-aminobutyric acid (GABA), the major inhibitory neurotransmitter in the central nervous system. GAD is the rate-limiting enzyme in the biosynthesis of GABA from L-glutamic acid, and it is used extensively as a marker for GABAergic neurons and synaptic terminals. GAD has been observed in pancreatic islet cells and identified as an autoantigen associated with the development of insulin-dependent diabetes mellitus.
Product Categories/Family for anti-Glutamic Acid Decarboxylase antibody
NCBI and Uniprot Product Information
NCBI GI #
NCBI Official Full Name
glutamic acid decarboxylase
Uniprot Description
GAD2: Catalyzes the production of GABA. Homodimer. Belongs to the group II decarboxylase family.
Protein type: Other Amino Acids Metabolism - taurine and hypotaurine; Carbohydrate Metabolism - butanoate; EC 4.1.1.15; Lyase; Amino Acid Metabolism - alanine, aspartate and glutamate; Other Amino Acids Metabolism - beta-alanine
Chromosomal Location of Human Ortholog: 10p11.23
Cellular Component: Golgi membrane; presynaptic membrane; synaptic vesicle membrane; axon; perinuclear region of cytoplasm; plasma membrane; cytosol; cell junction
Molecular Function: protein binding; glutamate binding; glutamate decarboxylase activity; protein heterodimerization activity; pyridoxal phosphate binding
Biological Process: response to drug; synaptic transmission; glutamate decarboxylation to succinate; neurotransmitter secretion; neurotransmitter biosynthetic process
Similar Products
Product Notes
The Glutamic Acid Decarboxylase (Catalog #AAA607899) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Glutamic Acid Decarboxylase (GAD) reacts with Rat and may cross-react with other species as described in the data sheet. AAA Biotech's Glutamic Acid Decarboxylase can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC). Suitable for use in Western Blot and Immunohistochemistry. Dilution: Immunohistochemistry: 1ug/ml. Researchers should empirically determine the suitability of the Glutamic Acid Decarboxylase for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Glutamic Acid Decarboxylase, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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