Mouse anti-Mouse, Rat Bassoon Monoclonal Antibody | anti-BSN antibody

Bassoon

Cat. Num. AAA601601

• Gene Names: BSN; ZNF231; BSN
• Reactivity: Mouse, Rat
• Applications: Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry
• Purity: Affinity Purified; Purified by Protein G affinity chromatography.
• Synonyms: Bassoon; Monoclonal Antibody; Anti -Bassoon; anti-BSN antibody

• Host: Mouse
• Reactivity: Mouse, Rat
• Clonality: Monoclonal
• Isotype: IgG2a
• Clone Number: 8J289
• Specificity: Recognizes rat bassoon at ~400kD. Additional bands between 97kD and 400kD haev been identified and are reported to be proteolytic degradation products of bassoon. Species Crossreactivity: mouse.
• Purity/Purification: Affinity Purified; Purified by Protein G affinity chromatography.
• Form/Format: Supplied as a liquid in PBS, 0.09% sodium azide, 50% glycerol

• Applicable Applications for anti-BSN antibody: Western Blot (WB), Immunoprecipitation (IP), Immunohistochemistry (IHC), Immunocytochemistry (ICC)
• Application Notes: Suitable for use in Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry.; Dilution: Immunocytochemistry: 1:400; Immunohistochemistry: Paraffin sections; Immunoprecipitation: 2.5ug; Western Blot: 1:500
• Immunogen: Recombinant fusion protein corresponding to aa738-1035 of rat bassoon expressed as a in E.coli.
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20 degree C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Bassoon localization in the rat brain cerebellar molecular layer using MBS601601.)

Related Product Information for anti-BSN antibody

Bassoon is a 420kD protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle (1-3). Bassoon does not belong to any known protein families. It has been found in rat and mouse with sequence identity of the two proteins at 96%. The human BASSOON gene has recently been cloned and localized (3). Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntingtin, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to colocalize with GABA (A) and glutamate (GluR1) receptors. Another presynaptic protein, Piccolo was found to colocalize with Bassoon in cultured hippocampal neurons. These observations suggested that they serve specific functions at synaptic junctions and may be involved in organization of the cytoskeleton at the site of neurotransmitter release.

Product Categories/Family for anti-BSN antibody

Antibodies; Abs to Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 3928704
• NCBI GeneID: 8927
• UniProt Accession #: Q9UPA5; O43161; Q7LGH3
• Molecular Weight: 416,469 Da
• NCBI Official Full Name: Bassoon protein
• NCBI Official Synonym Full Names: bassoon (presynaptic cytomatrix protein)
• NCBI Official Symbol: BSN
• NCBI Official Synonym Symbols: ZNF231; BSN
• NCBI Protein Information: protein bassoon; zinc finger protein 231; neuronal double zinc finger protein
• UniProt Protein Name: Protein bassoon
• Protein Family: Protein bassoon
• UniProt Gene Name: BSN
• UniProt Synonym Gene Names: KIAA0434; ZNF231
• UniProt Entry Name: BSN_HUMAN

NCBI Description

Neurotransmitters are released from a specific site in the axon terminal called the active zone, which is composed of synaptic vesicles and a meshwork of cytoskeleton underlying the plasma membrane. The protein encoded by this gene is thought to be a scaffolding protein involved in organizing the presynaptic cytoskeleton. The gene is expressed primarily in neurons in the brain. A similar gene product in rodents is concentrated in the active zone of axon terminals and tightly associated with cytoskeletal structures, and is essential for regulating neurotransmitter release from a subset of synapses. [provided by RefSeq]

Uniprot Description

BSN: involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses. Interacts with CAST1, RIMS1 and UNC13A. Part of a complex consisting of ERC2, RIMS1 and BSN. Localized to the active zone of presynaptic density. Mice lacking functional BSN showed a reduced excitability attributed to inactivation of a fraction of brain glutamatergic synapses. At these synapses, vesicles are clustered and docked in normal numbers, but are unable to fuse. In retina, mutants lacking functional BSN showed normal retinal anatomy, but synapses lacked anchoring of the photoreceptor ribbon to the presynaptic active zone resulting in impaired photoreceptor synaptic transmission. Two alternatively spliced isoforms have been reported.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: axon; postsynaptic density; dendrite; presynaptic active zone; excitatory synapse; cell junction; nucleus

Molecular Function: metal ion binding

Biological Process: synaptic transmission