Rabbit Bassoon Polyclonal Antibody | anti-BSN antibody

Bassoon Antibody

Cat. Num. AAA806552

• Gene Names: BSN; ZNF231
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunohistochemistry
• Synonyms: Bassoon; Polyclonal Antibody; Bassoon Antibody; BSN; ZNF231; Neuronal double zine finger protein; anti-BSN antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Form/Format: Protein G Purified
• Concentration: 1mg/mL (varies by lot)
• Sequence Length: 3926

• Applicable Applications for anti-BSN antibody: Western Blot (WB), Immunohistochemistry (IHC)
• Application Notes: 1:1000 (WB)
• Certificate of Analysis: 1ug/mL of SPC-198 was sufficient for detection of Bassoon in 10ug of rat brain tissue lysate by colorimetric immunoblot analysis using goat ant rabbit IgG:HRP as the secondary antibody.
• Conjugate: Unconjugated
• Storage Buffer: PBS pH7.4, 50% glycerol, 0.09% sodium azide
• Preparation and Storage: -20 degree C

Immunohistochemistry (IHC)

(Immunohistochemistry analysis using Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198). Tissue: Muscle. Species: Mouse. Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198) at 1:400. Secondary Antibody: Alexa Fluor 488 Goat Anti-Rabbit. Counterstain: BTX (red). Localization: Selective staining of the NMJ.)

Western Blot (WB)

(Western blot analysis of Mouse, Rat brain cell lysates showing detection of Bassoon protein using Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198). Primary Antibody: Rabbit Anti-Bassoon Polyclonal Antibody (SPC-198) at 1:1000.)

Related Product Information for anti-BSN antibody

Background Info: Detects ~420kDa, corresponding to the molecular mass of Bassoon. Multiple isoforms can be detected. No cross-reactivity to Piccolo.

Scientific Background: Bassoon (1) is a 420 kDa protein that is a localized at the presynaptic nerve terminals and is believed to play a role in the structural and functional organization of the synaptic vesicle cycle. Bassoon is predicted to contain two double-zinc fingers, three coiled-coil regions, and two polyglutamine domains. The polyglutamine domains in the C-terminus are of interest, since it is known that for some human proteins, such as Huntington, abnormal amplification of this region can cause late-onset neurodegeneration. Bassoon is concentrated at sites opposite to postsynaptic densities in synaptic terminals and in cultured neurons, it is found to co-localize with GABA (A) and glutamate (GluR1) receptors.

Product Categories/Family for anti-BSN antibody

Neuroscience

References

1. Anna Dondzillo et al., (2010) J. Comp Neur, 518(7): 1008-1029.

NCBI and Uniprot Product Information

• NCBI GI #: 91208420
• NCBI GeneID: 8927
• NCBI Accession #: NP_003449.2
• NCBI GenBank Nucleotide #: NM_003458.3
• UniProt Accession #: Q9UPA5; O43161; Q7LGH3
• Molecular Weight: 416,469 Da
• NCBI Official Full Name: protein bassoon
• NCBI Official Synonym Full Names: bassoon presynaptic cytomatrix protein
• NCBI Official Symbol: BSN
• NCBI Official Synonym Symbols: ZNF231
• NCBI Protein Information: protein bassoon; zinc finger protein 231; neuronal double zinc finger protein; bassoon (presynaptic cytomatrix protein)
• UniProt Protein Name: Protein bassoon
• Protein Family: Protein bassoon
• UniProt Gene Name: BSN
• UniProt Synonym Gene Names: KIAA0434; ZNF231
• UniProt Entry Name: BSN_HUMAN

NCBI Description

Neurotransmitters are released from a specific site in the axon terminal called the active zone, which is composed of synaptic vesicles and a meshwork of cytoskeleton underlying the plasma membrane. The protein encoded by this gene is thought to be a scaffolding protein involved in organizing the presynaptic cytoskeleton. The gene is expressed primarily in neurons in the brain. A similar gene product in rodents is concentrated in the active zone of axon terminals and tightly associated with cytoskeletal structures, and is essential for regulating neurotransmitter release from a subset of synapses. [provided by RefSeq, Jul 2008]

Uniprot Description

BSN: involved in the organization of the cytomatrix at the nerve terminals active zone (CAZ) which regulates neurotransmitter release. Essential in regulated neurotransmitter release from a subset of brain glutamatergic synapses. Involved in the formation of the retinal photoreceptor ribbon synapses. Interacts with CAST1, RIMS1 and UNC13A. Part of a complex consisting of ERC2, RIMS1 and BSN. Localized to the active zone of presynaptic density. Mice lacking functional BSN showed a reduced excitability attributed to inactivation of a fraction of brain glutamatergic synapses. At these synapses, vesicles are clustered and docked in normal numbers, but are unable to fuse. In retina, mutants lacking functional BSN showed normal retinal anatomy, but synapses lacked anchoring of the photoreceptor ribbon to the presynaptic active zone resulting in impaired photoreceptor synaptic transmission. Two alternatively spliced isoforms have been reported.

Protein type: Cytoskeletal

Chromosomal Location of Human Ortholog: 3p21.31

Cellular Component: axon; dendrite; postsynaptic density; presynaptic active zone; excitatory synapse; nucleus; cell junction

Molecular Function: metal ion binding

Biological Process: synaptic transmission