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Testing Data (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

Mouse BACE1 Monoclonal Antibody | anti-BACE1 antibody

BACE1 (beta-site APP-cleaving Enzyme 1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149) (Biotin)

Gene Names
BACE1; ASP2; BACE; HSPC104
Applications
Immunohistochemistry, Western Blot
Purity
Purified
Synonyms
BACE1; Monoclonal Antibody; BACE1 (beta-site APP-cleaving Enzyme 1; ASP2; BACE; FLJ90568; HSPC104; KIAA1149) (Biotin); beta-site APP-cleaving Enzyme 1; KIAA1149; anti-BACE1 antibody
Ordering
For Research Use Only!
Host
Mouse
Clonality
Monoclonal
Isotype
IgG1,k
Clone Number
2C1
Specificity
Recognizes BACE1.
Purity/Purification
Purified
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Biotin.
Sequence Length
501
Applicable Applications for anti-BACE1 antibody
Immunohistochemistry (IHC), Western Blot (WB), In situ Proximity Ligation Assay (Cell)
Application Notes
Applications are based on unconjugated antibody.
Immunogen
BACE1 (AAH65492, 22aa-501aa) full-length recombinant protein with GST tag. MW of the GST tag alone is 26kD.
Immunogen Sequence
TQHGIRLPLRSGLGGAPLGLRLPRETDEEPEEPGRRGSFVEMVDNLRGKSGQGYYVEMTVGSPPQTLNILVDTGSSNFAVGAAPHPFLHRYYQRQLSSTYRDLRKGVYVPYTQGKWEGELGTDLVSIPHGPNVTVRANIAAITESDKFFINGSNWEGILGLAYAEIARPDDSLEPFFDSLVKQTHVPNLFSLQLCGAGFPLNQSEVLASVGGSMIIGGIDHSLYTGSLWYTPIRREWYYEVIIVRVEINGQDLKMDCKEYNYDKSIVDSGTTNLRLPKKVFEAAVKSIKAASSTEKFPDGFWLGEQLVCWQAGTTPWNIFPVISLYLMGEVTNQSFRITILPQQYLRPVEDVATSQDDCYKFAISQSSTGTVMGAVIMEGFYVVFDRARKRIGFAVSACHVHDEFRTAAVEGPFVTLDMEDCGYNIPQTDESTLMTIAYVMAAICALFMLPLCLMVCQWRCLRCLRQQHDDFADDISLLK
Conjugate
Biotin
Preparation and Storage
Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Testing Data

(Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

Testing Data (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

Immunohistochemistry (IHC)

(Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

Immunohistochemistry (IHC) (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

Testing Data

(Detection limit for recombinant GST tagged BACE1 is approximately 0.03ng/ml as a capture antibody.)

Testing Data (Detection limit for recombinant GST tagged BACE1 is approximately 0.03ng/ml as a capture antibody.)

Testing Data

(Proximity Ligation Analysis of protein-protein interactions between MMP2 and BACE1. HeLa cells were stained with anti-MMP2 rabbit purified polyclonal 1:1200 and anti-BACE1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Testing Data (Proximity Ligation Analysis of protein-protein interactions between MMP2 and BACE1. HeLa cells were stained with anti-MMP2 rabbit purified polyclonal 1:1200 and anti-BACE1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Western Blot (WB)

(Western Blot analysis of BACE1 expression in transfected 293T cell line by BACE1 monoclonal antibody (M01), clone 2C1.Lane 1: BACE1 transfected lysate (52.9 KDa).Lane 2: Non-transfected lysate.)

Western Blot (WB) (Western Blot analysis of BACE1 expression in transfected 293T cell line by BACE1 monoclonal antibody (M01), clone 2C1.Lane 1: BACE1 transfected lysate (52.9 KDa).Lane 2: Non-transfected lysate.)

Western Blot (WB)

(BACE1 monoclonal antibody (M02), clone 2C1. Western Blot analysis of BACE1 expression in rat testis.)

Western Blot (WB) (BACE1 monoclonal antibody (M02), clone 2C1. Western Blot analysis of BACE1 expression in rat testis.)
Related Product Information for anti-BACE1 antibody
Cerebral deposition of amyloid beta peptide is an early and critical feature of Alzheimer's disease. Amyloid beta peptide is generated by proteolytic cleavage of amyloid precursor protein (APP) by two proteases, one of which is the protein encoded by this gene. The encoded protein, a member of the peptidase A1 protein family, is a type I integral membrane glycoprotein and aspartic protease that is found mainly in the Golgi. Four transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq]
Product Categories/Family for anti-BACE1 antibody

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Official Full Name
Beta-site APP-cleaving enzyme 1
NCBI Official Synonym Full Names
beta-secretase 1
NCBI Official Symbol
BACE1
NCBI Official Synonym Symbols
ASP2; BACE; HSPC104
NCBI Protein Information
beta-secretase 1
Protein Family

NCBI Description

This gene encodes a member of the peptidase A1 family of aspartic proteases. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protease. This transmembrane protease catalyzes the first step in the formation of amyloid beta peptide from amyloid precursor protein. Amyloid beta peptides are the main constituent of amyloid beta plaques, which accumulate in the brains of human Alzheimer's disease patients. [provided by RefSeq, Nov 2015]

Research Articles on BACE1

Similar Products

Product Notes

The BACE1 (Catalog #AAA6174670) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's BACE1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Western Blot (WB), In situ Proximity Ligation Assay (Cell). Applications are based on unconjugated antibody. Researchers should empirically determine the suitability of the BACE1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "BACE1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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