Mouse Brain-derived neurotrophic factor ELISA Kit | Bdnf elisa kit

Mouse Brain-derived neurotrophic factor ELISA Kit

Cat. Num. AAA9424908

• Reactivity: Mouse
• Synonyms: Brain-derived neurotrophic factor; Mouse Brain-derived neurotrophic factor ELISA Kit; BDNF; Bdnf; Bdnf elisa kit

• Reactivity: Mouse
• Specificity: This assay recognizes recombinant and natural mouseBDNF. No significant cross-reactivity or interference was observed.

• Samples: Serum, plasma,tissue homogenates and cell culture supernates and other biological fluids
• Detection Range: 0.156 ng/ml -10ng/ml
• Sensitivity: <0.08 ng/ml
• Preparation and Storage: Store at 4 degree C

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Bdnf elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of mouse bdnf concentrations in serum, plasma,tissue homogenates and cell culture supernates and other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to BDNF. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific forBDNF and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain BDNF, biotinconjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of BDNF in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 114326457
• NCBI GeneID: 12064
• NCBI Accession #: NP_001041604.1
• NCBI GenBank Nucleotide #: NM_001048139.1
• UniProt Accession #: P21237
• Molecular Weight: 28,123 Da
• NCBI Official Full Name: brain-derived neurotrophic factor isoform 2 preproprotein
• NCBI Official Synonym Full Names: brain derived neurotrophic factor
• NCBI Official Symbol: Bdnf
• NCBI Protein Information: brain-derived neurotrophic factor
• UniProt Protein Name: Brain-derived neurotrophic factor
• Protein Family: Brain-derived neurotrophic factor
• UniProt Gene Name: Bdnf
• UniProt Synonym Gene Names: BDNF

NCBI Description

The protein encoded by this gene is a member of the nerve growth factor family. It is involved in the growth, differentiation and survival of specific types of developing neurons both in the central nervous system (CNS) and the peripheral nervous system. It is also involved in regulating synaptic plasticity in the CNS. Expression of a similar gene in human is reduced in both Alzheimer's and Huntington disease patients. Alternative splicing results in multiple transcript variants encoding different isoforms that may undergo similar processing to generate mature protein. [provided by RefSeq, Oct 2015]

Uniprot Description

BDNF: During development, promotes the survival and differentiation of selected neuronal populations of the peripheral and central nervous systems. Participates in axonal growth, pathfinding and in the modulation of dendritic growth and morphology. Major regulator of synaptic transmission and plasticity at adult synapses in many regions of the CNS. The versatility of BDNF is emphasized by its contribution to a range of adaptive neuronal responses including long-term potentiation (LTP), long-term depression (LTD), certain forms of short-term synaptic plasticity, as well as homeostatic regulation of intrinsic neuronal excitability. Defects in BDNF are a cause of congenital central hypoventilation syndrome (CCHS); also known as congenital failure of autonomic control or Ondine curse. CCHS is a rare disorder characterized by abnormal control of respiration in the absence of neuromuscular or lung disease, or an identifiable brain stem lesion. A deficiency in autonomic control of respiration results in inadequate or negligible ventilatory and arousal responses to hypercapnia and hypoxemia. CCHS is frequently complicated with neurocristopathies such as Hirschsprung disease that occurs in about 16% of CCHS cases. Belongs to the NGF-beta family. 5 isoforms of the human protein are produced by alternative promoter.

Protein type: Cell development/differentiation; Cytokine; Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 2 E3|2 56.63 cM

Cellular Component: axon; cell soma; cytoplasm; cytoplasmic vesicle; dendrite; extracellular space; mitochondrial crista; mitochondrion; nuclear speck; perikaryon; perinuclear region of cytoplasm; secretory granule; synaptic vesicle; terminal button

Molecular Function: growth factor activity; neurotrophin TRKB receptor binding; protein binding

Biological Process: axon extension; axon guidance; axon target recognition; behavioral fear response; circadian rhythm; collateral sprouting; dendrite development; fear response; feeding behavior; gamma-aminobutyric acid signaling pathway; glutamate secretion; inner ear development; learning; learning and/or memory; mechanoreceptor differentiation; mitochondrial electron transport, NADH to ubiquinone; negative regulation of apoptosis; negative regulation of neuroblast proliferation; negative regulation of neuron apoptosis; negative regulation of striated muscle development; negative regulation of synaptic transmission, GABAergic; nerve development; nervous system development; neuron recognition; positive regulation of axon extension; positive regulation of collateral sprouting; positive regulation of long-term neuronal synaptic plasticity; positive regulation of neuron differentiation; positive regulation of peptidyl-serine phosphorylation; positive regulation of synaptogenesis; positive regulation of transcription factor activity; regeneration; regulation of axon extension; regulation of collateral sprouting; regulation of excitatory postsynaptic membrane potential; regulation of inhibitory postsynaptic membrane potential; regulation of long-term neuronal synaptic plasticity; regulation of metabolic process; regulation of neuron apoptosis; regulation of retinal cell programmed cell death; regulation of short-term neuronal synaptic plasticity; regulation of synaptic plasticity; response to drug; synaptogenesis; transmembrane receptor protein tyrosine kinase signaling pathway; ureteric bud development