ELISA (EIA)
(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human B7-1 protein, hFc tagged protein can bind Rabbit anti-B7-1 monoclonal antibody (clone: DM111) in a linear range of 0.2-80 ng/ml.)
Rabbit anti-Human B7-1 Monoclonal Antibody | anti-B7-1 antibody
Anti-B7-1 antibody (DM111), Rabbit mAb
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Reactivity
Human
Applications
ELISA, Flow Cytometry, Functional Assay
Purity
Purified from cell culture supernatant by affinity chromatography
Synonyms
B7-1; Monoclonal Antibody; Anti-B7-1 antibody (DM111); Rabbit mAb; CD80; B7; B7.1; BB1; CD28LG; CD28LG1; LAB7; anti-B7-1 antibody
Host
Rabbit
Reactivity
Human
Clonality
Monoclonal
Isotype
Rabbit IgG
Clone Number
DM111
Purity/Purification
Purified from cell culture supernatant by affinity chromatography
Form/Format
Lyophilized from sterile PBS, pH7.4. Normally 5%-8% trehalose is added as protectants before lyophilization. 0.1% Procline 300
Applicable Applications for anti-B7-1 antibody
ELISA (EIA), Flow Cytometry (FC/FACS)
Application Notes
ELISA: 1:5000-10,000
FC/FACS: 1:100
FC/FACS: 1:100
Immunogen
Recombinant human B7-1 (Val35-Asn242) produced by using human HEK293 cells
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -20 degree C for 12 months (Avoid repeated freezing and thawing)
ELISA (EIA)
(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human B7-1 protein, hFc tagged protein can bind Rabbit anti-B7-1 monoclonal antibody (clone: DM111) in a linear range of 0.2-80 ng/ml.)
ELISA (EIA)
(Figure 1. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human B7-1 protein, hFc tagged protein can bind Rabbit anti-B7-1 monoclonal antibody (clone: DM111) in a linear range of 0.2-80 ng/ml.)
Flow Cytometry (FC/FACS)
(Figure 2. Flow cytometry analysis with Anti-B7-1 (DM111) on Expi293 cells transfected with human B7-1(Blue histogram) or Expi293 transfected with irrelevant protein(Red histogram).)
Flow Cytometry (FC/FACS)
(Figure 2. Flow cytometry analysis with Anti-B7-1 (DM111) on Expi293 cells transfected with human B7-1(Blue histogram) or Expi293 transfected with irrelevant protein(Red histogram).)
Related Product Information for anti-B7-1 antibody
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
Molecular Weight
18,761 Da
NCBI Official Full Name
T-lymphocyte activation antigen CD80
NCBI Official Synonym Full Names
CD80 molecule
NCBI Official Symbol
CD80
NCBI Official Synonym Symbols
B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
NCBI Protein Information
T-lymphocyte activation antigen CD80
UniProt Protein Name
T-lymphocyte activation antigen CD80
UniProt Gene Name
CD80
UniProt Synonym Gene Names
CD28LG; CD28LG1; LAB7; B7
NCBI Description
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]
Uniprot Description
Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.
Research Articles on B7-1
Similar Products
Product Notes
The B7-1 cd80 (Catalog #AAA188636) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Anti-B7-1 antibody (DM111), Rabbit mAb reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's B7-1 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow Cytometry (FC/FACS). ELISA: 1:5000-10,000 FC/FACS: 1:100. Researchers should empirically determine the suitability of the B7-1 cd80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "B7-1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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