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SDS-Page (Figure 1. Human B7-1 Protein, hFc Tag on SDS-PAGE under reducing condition.)

B7-1 recombinant protein

Recombinant human B7-1 protein with C-terminal human Fc tag

Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Applications
Western Blot, ELISA
Purity
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Synonyms
B7-1; Recombinant human B7-1 protein with C-terminal human Fc tag; Human B7-1 Protein; hFc Tag; CD80;B7;B7-1;B7.1;BB1;CD28LG;CD28LG1;LAB7; B7-1 recombinant protein
Ordering
For Research Use Only!
Host
HEK293 cells
Purity/Purification
The purity of the protein is greater than 95% as determined by SDS-PAGE and Coomassie blue staining.
Form/Format
Lyophilized from sterile PBS, pH 7.4. Normally 5%-92% trehalose is added as protectants before lyophilization.
Sequence
B7-1(Val35-Asn242)+hFc(Glu99-Ala330)
Applicable Applications for B7-1 recombinant protein
Western Blot (WB), ELISA (EIA)
Source
Human
Conjugation
C-Human Fc tag
Reconstitution
Reconstitute with deionized water
Preparation and Storage
Store at -80 degree C for 12 months (Avoid repeated freezing and thawing)
Shipping: Blue Ice

SDS-Page

(Figure 1. Human B7-1 Protein, hFc Tag on SDS-PAGE under reducing condition.)

SDS-Page (Figure 1. Human B7-1 Protein, hFc Tag on SDS-PAGE under reducing condition.)

ELISA (EIA)

(Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human B7-1, hFc tagged protein can bind Human CTLA4, mFc-His tagged protein in a linear range of 0.048-2.094 ng/ml.)

ELISA (EIA) (Figure 2. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human B7-1, hFc tagged protein can bind Human CTLA4, mFc-His tagged protein in a linear range of 0.048-2.094 ng/ml.)

ELISA (EIA)

(Figure 3. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD28, mFc-His tagged protein can bind Human B7-1, hFc tagged protein in a linear range of 125-4000 ng/ml.)

ELISA (EIA) (Figure 3. ELISA plate pre-coated by 2 ug/ml (100 ul/well) Human CD28, mFc-His tagged protein can bind Human B7-1, hFc tagged protein in a linear range of 125-4000 ng/ml.)
Related Product Information for B7-1 recombinant protein
The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
941
UniProt Accession #
Molecular Weight
18,761 Da
NCBI Official Full Name
T-lymphocyte activation antigen CD80
NCBI Official Synonym Full Names
CD80 molecule
NCBI Official Symbol
CD80
NCBI Official Synonym Symbols
B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
NCBI Protein Information
T-lymphocyte activation antigen CD80
UniProt Protein Name
T-lymphocyte activation antigen CD80
UniProt Gene Name
CD80
UniProt Synonym Gene Names
CD28LG; CD28LG1; LAB7; B7

NCBI Description

The protein encoded by this gene is a membrane receptor that is activated by the binding of CD28 or CTLA-4. The activated protein induces T-cell proliferation and cytokine production. This protein can act as a receptor for adenovirus subgroup B and may play a role in lupus neuropathy. [provided by RefSeq, Aug 2011]

Uniprot Description

Involved in the costimulatory signal essential for T-lymphocyte activation. T-cell proliferation and cytokine production is induced by the binding of CD28, binding to CTLA-4 has opposite effects and inhibits T-cell activation.

Research Articles on B7-1

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Product Notes

The B7-1 cd80 (Catalog #AAA188152) is a Recombinant Protein produced from HEK293 cells and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's B7-1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). Researchers should empirically determine the suitability of the B7-1 cd80 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: B7-1(Val35 -Asn242)+h Fc(Glu99-A la330). It is sometimes possible for the material contained within the vial of "B7-1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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