Western Blot (WB)
(WB: Rat brain microsomal preparation was probed with MBS610854 (2ug/ml).)
Rabbit KIF21A Polyclonal Antibody | anti-KIF21A antibody
KIF21A (Kinesin Family Member 21A) (AP)
Reactivity
Mouse, Human, Rat
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Synonyms
KIF21A; Polyclonal Antibody; KIF21A (Kinesin Family Member 21A) (AP); anti-KIF21A antibody
Host
Rabbit
Reactivity
Mouse, Human, Rat
Clonality
Polyclonal
Isotype
IgG
Specificity
Recognizes mouse KIF21A, Mr ~180kD. An unknown protein detected at Mr ~70kD was also competed by the immunizing peptide. Species Crossreactivity: rat and human.
Purity/Purification
Purified by Protein A Affinity Chromatography.
Form/Format
Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Alkaline Phosphatase.
Applicable Applications for anti-KIF21A antibody
Western Blot (WB)
Application Notes
Applications are based on unconjugated antibody.
WB: 1-2ug/ml detects KIF21A in a rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
WB: 1-2ug/ml detects KIF21A in a rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Immunogen
Synthetic peptide corresponding to amino acids 948-963 (CKRREKLSKRREKIVKE) of mouse KIF21A with an N-terminal cysteine added for conjugation purposes (KLH coupled).
Conjugate
AP
Note
Preservative Free
Preparation and Storage
May be stored at 4 degree C for short-term only. For long-term storage, store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Western Blot (WB)
(WB: Rat brain microsomal preparation was probed with MBS610854 (2ug/ml).)
Western Blot (WB)
(WB: Rat brain microsomal preparation was probed with MBS610854 (2ug/ml).)
Product Categories/Family for anti-KIF21A antibody
References
1. Nakamura N., et al., J. Biochem. (Tokyo) 132: 483-491, 2002 2. Marszalek J.R., et al., J. Cell Biol. 145: 469-479, 1999.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
186,536 Da
NCBI Official Full Name
kinesin-like protein KIF21A isoform 1
NCBI Official Synonym Full Names
kinesin family member 21A
NCBI Official Symbol
Kif21a
NCBI Protein Information
kinesin-like protein KIF21A; N-5 kinesin
UniProt Protein Name
Kinesin-like protein KIF21A
Protein Family
UniProt Gene Name
Kif21a
UniProt Synonym Gene Names
Kiaa1708
UniProt Entry Name
KI21A_MOUSE
Similar Products
Product Notes
The KIF21A kif21a (Catalog #AAA6484338) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The KIF21A (Kinesin Family Member 21A) (AP) reacts with Mouse, Human, Rat and may cross-react with other species as described in the data sheet. AAA Biotech's KIF21A can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB). Applications are based on unconjugated antibody. WB: 1-2ug/ml detects KIF21A in a rat brain microsomal preparation. Rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and probed with. Proteins were visualized using a goat anti-rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system. Researchers should empirically determine the suitability of the KIF21A kif21a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "KIF21A, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
