Mouse ATPase beta 1, Na, K Monoclonal Antibody | anti-ATP1B1 antibody

ATPase beta 1, Na, K

Cat. Num. AAA601891

• Reactivity: Porcine, Rabbit, Rat
• Applications: ELISA
• Purity: Ascites; Ascities
• Synonyms: ATPase beta 1; Na; K; Monoclonal Antibody; Anti -ATPase beta 1; anti-ATP1B1 antibody

• Host: Mouse
• Reactivity: Porcine, Rabbit, Rat
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 3C115
• Specificity: Specific for Na,K-ATPase beta 1 subunit Species crossreactivity: Human, porcine, rat, bovine, rabbit, and canine. Does not crossreact with mouse. Results on CHO have been mixed.
• Purity/Purification: Ascites; Ascities
• Form/Format: Supplied as a liquid, ascites, 0.2% sodium azide.

• Applicable Applications for anti-ATP1B1 antibody: ELISA (EL/EIA)
• Application Notes: Suitable for use in Western Blot.; Dilution: Western Blot: 1:2500 (ECL). Demonstates a very broad banding pattern, typical of glycosylated proteins, at 35kD. May see multiple bands migrating between 35-55kD.; Optimal dilutions to be determined by researcher.
• Immunogen: Purified Na, K-ATPase from rabbit renal outer medulla
• Preparation and Storage: May be stored at 4 degree C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20 degree C. Aliquots are stable for at least 12 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.

Related Product Information for anti-ATP1B1 antibody

Na,K-ATPase is an integral membrane protein complex that hydrolyzes ATP to maintain the transmembrane gradients of Na+ and K+ found in most mammalian cells. The enzyme is comprised of an alpha and Beta subunit. The á-polypeptide has been shown to be the catalytically active subunit, whereas the Beta-polypeptide is glycosylated and appears to be necessary for the assembly and transport of the sodium pump to the plasma membrane. Na,K-ATPase Beta-1 subunit is expressed, exclusively, in epithelial cells.

Product Categories/Family for anti-ATP1B1 antibody

Antibodies; Abs to Enzymes; ATPase

NCBI and Uniprot Product Information

• NCBI GI #: 146741430
• NCBI GeneID: 457497
• UniProt Accession #: A5A6J8
• Molecular Weight: 35,075 Da
• NCBI Official Full Name: ATPase, Na+/K+ transporting, beta 1 polypeptide, transcript variant 1
• NCBI Official Symbol: ATP1B1
• NCBI Protein Information: sodium/potassium-transporting ATPase subunit beta-1; Na+/K+ -ATPase beta 1 subunit; sodium/potassium-dependent ATPase subunit beta-1
• UniProt Protein Name: Sodium/potassium-transporting ATPase subunit beta-1
• UniProt Gene Name: ATP1B1
• UniProt Entry Name: AT1B1_PANTR

Uniprot Description

Function: This is the non-catalytic component of the active enzyme, which catalyzes the hydrolysis of ATP coupled with the exchange of Na+ and K+ ions across the plasma membrane. The beta subunit regulates, through assembly of alpha/beta heterodimers, the number of sodium pumps transported to the plasma membrane

Subunit structure: Composed of three subunits: alpha (catalytic), beta and gamma. Interacts with NKAIN1, NKAIN2 and NKAIN4

Subcellular location: Membrane; Single-pass type II membrane protein

Sequence similarities: Belongs to the X(+)/potassium ATPases subunit beta family.