Rabbit ATP5A1 Polyclonal Antibody | anti-ATP5A1 antibody

ATP5A1 antibody

Cat. Num. AAA9414984

• Gene Names: ATP5A1; OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m
• Reactivity: Human, Mouse, Rat
• Applications: Western Blot, Immunofluorescence
• Purity: Antibodies were purified by affinity purification using immunogen.
• Synonyms: ATP5A1; Polyclonal Antibody; ATP5A1 antibody; OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2; HEL-S-123m; anti-ATP5A1 antibody

• Host: Rabbit
• Reactivity: Human, Mouse, Rat
• Clonality: Polyclonal
• Specificity: The antibody detects endogenous level of total ATP5A1 antibody.
• Purity/Purification: Antibodies were purified by affinity purification using immunogen.
• Form/Format: Supplied at 1.0mg/mL in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1.0 mg/ml (varies by lot)
• Sequence Length: 503

• Applicable Applications for anti-ATP5A1 antibody: Western Blot (WB), Immunofluorescence (IF)
• Application Notes: Western blotting: 1:500 - 1:2000; Immunofluorescence: 1:50 - 1:200
• Immunogen Type: Recombinant Protein
• Immunogen Description: Recombinant protein of human ATP5A1.
• Target Name: ATP5A1
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis of extracts of various cell lines, using ATP5A1 antibody.)

Immunofluorescence (IF)

(Immunofluorescence analysis of U20S cell using ATP5A1 antibody. Blue: DAPI for nuclear staining.)

Related Product Information for anti-ATP5A1 antibody

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16.

Product Categories/Family for anti-ATP5A1 antibody

Total protein Ab

NCBI and Uniprot Product Information

• NCBI GI #: 50345982
• NCBI GeneID: 498
• NCBI Accession #: NP_001001935.1
• NCBI GenBank Nucleotide #: NM_001001935.2
• UniProt Accession #: P25705; Q53XX6; Q8IXV2; Q96FB4; Q96HW2; Q96IR6; Q9BTV8; A8K092; B4DY56; K7ENP3
• Molecular Weight: 57,547 Da
• NCBI Official Full Name: ATP synthase subunit alpha, mitochondrial isoform c
• NCBI Official Synonym Full Names: ATP synthase, H+ transporting, mitochondrial F1 complex, alpha subunit 1, cardiac muscle
• NCBI Official Symbol: ATP5A1
• NCBI Official Synonym Symbols: OMR; ORM; ATPM; MOM2; ATP5A; hATP1; MC5DN4; ATP5AL2; COXPD22; HEL-S-123m
• NCBI Protein Information: ATP synthase subunit alpha, mitochondrial
• UniProt Protein Name: ATP synthase subunit alpha, mitochondrial
• UniProt Gene Name: ATP5A1
• UniProt Synonym Gene Names: ATP5A; ATP5AL2; ATPM
• UniProt Entry Name: ATPA_HUMAN

NCBI Description

This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, using an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel consists of three main subunits (a, b, c). This gene encodes the alpha subunit of the catalytic core. Alternatively spliced transcript variants encoding the different isoforms have been identified. Pseudogenes of this gene are located on chromosomes 9, 2, and 16. [provided by RefSeq, Mar 2012]

Uniprot Description

ATP5A1: Mitochondrial membrane ATP synthase (F(1)F(0) ATP synthase or Complex V) produces ATP from ADP in the presence of a proton gradient across the membrane which is generated by electron transport complexes of the respiratory chain. F-type ATPases consist of two structural domains, F(1) - containing the extramembraneous catalytic core, and F(0) - containing the membrane proton channel, linked together by a central stalk and a peripheral stalk. During catalysis, ATP synthesis in the catalytic domain of F(1) is coupled via a rotary mechanism of the central stalk subunits to proton translocation. Subunits alpha and beta form the catalytic core in F(1). Rotation of the central stalk against the surrounding alpha(3)beta(3) subunits leads to hydrolysis of ATP in three separate catalytic sites on the beta subunits. Subunit alpha does not bear the catalytic high-affinity ATP-binding sites. Belongs to the ATPase alpha/beta chains family.

Protein type: Transporter; Energy Metabolism - oxidative phosphorylation; EC 3.6.3.14; Membrane protein, integral; Mitochondrial; Hydrolase

Chromosomal Location of Human Ortholog: 18q21

Cellular Component: signalosome; membrane; mitochondrion; mitochondrial matrix; mitochondrial inner membrane; proton-transporting ATP synthase complex, catalytic core F(1); plasma membrane; mitochondrial proton-transporting ATP synthase complex

Molecular Function: protein binding; MHC class I protein binding; ATPase activity; hydrogen ion transporting ATP synthase activity, rotational mechanism; transmembrane transporter activity; hydrogen ion transporting ATPase activity, rotational mechanism; ATP binding

Biological Process: cellular metabolic process; embryonic development; ATP biosynthetic process; ATP hydrolysis coupled proton transport; negative regulation of endothelial cell proliferation; mitochondrial ATP synthesis coupled proton transport; lipid metabolic process

Disease: Combined Oxidative Phosphorylation Deficiency 22; Mitochondrial Complex V (atp Synthase) Deficiency, Nuclear Type 4